BerkiGEM2007-AustinConstructionFile20
From 2007.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | pcr CA1150F/G00101 on 9145-I716054 (575 bp) | + | pcr CA1150F/G00101 on 9145-I716054 (575 bp)<br> |
| - | Product is ADtemp1 | + | Product is ADtemp1<br> |
| - | Sub ADtemp1 into pTRC plasmid (Bgl2/XhoI) | + | Sub ADtemp1 into pTRC plasmid (Bgl2/XhoI)<br> |
| - | Product is ADtemp2 | + | Product is ADtemp2<br> |
| - | pcr CA1099F/D58 on ADtemp2 (3465 bp) | + | pcr CA1099F/D58 on ADtemp2 (3465 bp)<br> |
| - | Product is ADtemp3 | + | Product is ADtemp3<br> |
| - | _______________________________________________________ | + | _______________________________________________________<br> |
| - | Get biobrick of a TT somewhere, either from an already made plasmid or order the oligos below. | + | Get biobrick of a TT somewhere, either from an already made plasmid or order the oligos below. <br> |
| - | Call the alpha-TT construct ADtemp4 | + | Call the alpha-TT construct ADtemp4<br> |
| - | Pcr CA1154F/CA1099R on ADtemp4 (1607 +TT bp) | + | Pcr CA1154F/CA1099R on ADtemp4 (1607 +TT bp)<br> |
| - | Product is ADtemp5 | + | Product is ADtemp5<br> |
| - | ____________________________________________________________ | + | ____________________________________________________________<br> |
| - | Digest ADtemp3 (BsaI/BamHI) | + | Digest ADtemp3 (BsaI/BamHI) <br> |
| - | Digest ADtemp5 (BsaI/BglII) | + | Digest ADtemp5 (BsaI/BglII)<br> |
Ligate, product is: I716070 | Ligate, product is: I716070 | ||
| + | <br> | ||
Revision as of 08:09, 25 June 2007
pcr CA1150F/G00101 on 9145-I716054 (575 bp)
Product is ADtemp1
Sub ADtemp1 into pTRC plasmid (Bgl2/XhoI)
Product is ADtemp2
pcr CA1099F/D58 on ADtemp2 (3465 bp)
Product is ADtemp3
_______________________________________________________
Get biobrick of a TT somewhere, either from an already made plasmid or order the oligos below.
Call the alpha-TT construct ADtemp4
Pcr CA1154F/CA1099R on ADtemp4 (1607 +TT bp)
Product is ADtemp5
____________________________________________________________
Digest ADtemp3 (BsaI/BamHI)
Digest ADtemp5 (BsaI/BglII)
Ligate, product is: I716070
