BerkiGEM2007-AustinConstructionFile20

From 2007.igem.org

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pcr CA1150F/G00101 on 9145-I716054 (575 bp)<br>
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Digest 9145-bca1092 (Bgl2/EcoR1, 2206/3, Larger)<br>
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Product is ADtemp1<br>
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Digest I716053 (BamH1/EcoR1, 2063/444, Smaller)<br>
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Sub ADtemp1 into pTRC plasmid (Bgl2/XhoI)<br>
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Ligated product is I716070<br>
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Product is ADtemp2<br>
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pcr CA1099F/D58 on ADtemp2 (3465 bp)<br>
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Product is ADtemp3<br>
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_______________________________________________________<br>
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Pcr CA1154F/CA1099R on I716070 (1607 +TT bp)<br>
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Product is ADtemp5<br>
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____________________________________________________________<br>
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Digest ADtemp3 (BsaI/BamHI)    <br>
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Digest ADtemp5 (BsaI/BglII)<br>
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Ligate, product is: I716071
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<br>
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Latest revision as of 18:01, 25 June 2007

Digest 9145-bca1092 (Bgl2/EcoR1, 2206/3, Larger)
Digest I716053 (BamH1/EcoR1, 2063/444, Smaller)
Ligated product is I716070