BerkiGEM2007-AustinConstructionFile20

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Revision as of 18:01, 25 June 2007 by AustinDay (Talk | contribs)

pcr CA1150F/G00101 on 9145-I716054 (575 bp)
Product is ADtemp1
Sub ADtemp1 into pTRC plasmid (Bgl2/XhoI)
Product is ADtemp2
pcr CA1099F/D58 on ADtemp2 (3465 bp)
Product is ADtemp3
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Pcr CA1154F/CA1099R on I716070 (1607 +TT bp)
Product is ADtemp5
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Digest ADtemp3 (BsaI/BamHI)
Digest ADtemp5 (BsaI/BglII)
Ligate, product is: I716071