BerkiGEM2007-AustinConstructionFile21
From 2007.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
| + | Beta portion<br> | ||
pcr CA1154F/G00101 on 9145-I716054 (575 bp)<br> | pcr CA1154F/G00101 on 9145-I716054 (575 bp)<br> | ||
Product is ADtemp1<br> | Product is ADtemp1<br> | ||
| Line 6: | Line 7: | ||
Product is ADtemp3<br> | Product is ADtemp3<br> | ||
_______________________________________________________<br> | _______________________________________________________<br> | ||
| - | + | Alpha portion<br> | |
| - | Pcr CA1150F/CA1099R on I716070 ( | + | Pcr CA1150F/CA1099R on I716070 (1727 bp)<br> |
Product is ADtemp5<br> | Product is ADtemp5<br> | ||
____________________________________________________________<br> | ____________________________________________________________<br> | ||
Revision as of 18:05, 25 June 2007
Beta portion
pcr CA1154F/G00101 on 9145-I716054 (575 bp)
Product is ADtemp1
Sub ADtemp1 into pTRC plasmid (Bgl2/XhoI)
Product is ADtemp2
pcr CA1099F/D58 on ADtemp2 (3465 bp)
Product is ADtemp3
_______________________________________________________
Alpha portion
Pcr CA1150F/CA1099R on I716070 (1727 bp)
Product is ADtemp5
____________________________________________________________
Digest ADtemp3 (BsaI/BamHI)
Digest ADtemp5 (BsaI/BglII)
Ligate, product is: I716071
