From 2007.igem.org

Revision as of 19:00, 2 July 2007 (view source) AustinDay (Talk | contribs) ← Older edit		Revision as of 21:38, 2 July 2007 (view source) AustinDay (Talk | contribs) Newer edit →
Line 5:		Line 5:
	Beta: <br>		Beta: <br>
-	PCR CA1150F/D58 on Part Ptrc-I716080 <br>	+	PCR CA1099R/D58 on Part Ptrc-I716080 <br>
	Digest (Bsa1/BamH1) and ligate with alpha portion. <br>		Digest (Bsa1/BamH1) and ligate with alpha portion. <br>
	<br><br>		<br><br>
	Product is pTRC-I716071		Product is pTRC-I716071

---

Revision as of 21:38, 2 July 2007

Alpha:
PCR CA1150/CA1099R on I716070 (1727 Bp)
Digest (Bsa1/Bgl2) and ligate with beta portion.

Beta:

PCR CA1099R/D58 on Part Ptrc-I716080
Digest (Bsa1/BamH1) and ligate with alpha portion.


Product is pTRC-I716071