BerkiGEM2007-AustinConstructionFile21

From 2007.igem.org

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Alpha: <br>
Alpha: <br>
-
PCR CA1150/CA1099R on I716070 (1727 Bp)<br>
+
PCR CA1150/CA1099R on 9145-I716070 (1727 Bp)<br>
-
Digest (Bsa1/Bgl2) and ligate with beta portion.<br> <br>
+
Digest (Bsa1/Bgl2) (1686/Small, Larger) and ligate with beta portion.<br> <br>
Beta: <br>
Beta: <br>
-
PCR CA1150F/D58 on Part Ptrc-I716080 <br>
+
PCR CA1099F/G00101 on Part 9145-I716080 (1567 bp)<br>
-
Digest (Bsa1/BamH1) and ligate with alpha portion. <br>
+
Digest (Bsa1/BamH1) (1441/small, Larger) and ligate with alpha portion. <br>
<br><br>
<br><br>
-
Product is
+
Product is 9145-I716071

Latest revision as of 22:45, 10 July 2007

Alpha:
PCR CA1150/CA1099R on 9145-I716070 (1727 Bp)
Digest (Bsa1/Bgl2) (1686/Small, Larger) and ligate with beta portion.

Beta:

PCR CA1099F/G00101 on Part 9145-I716080 (1567 bp)
Digest (Bsa1/BamH1) (1441/small, Larger) and ligate with alpha portion.


Product is 9145-I716071