BerkiGEM2007-AustinConstructionFile23
From 2007.igem.org
(Difference between revisions)
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- | PCR ca1099F/AD0005R on 9145-I716053 -HbA alpha unmutated-<br> | + | PCR ca1099F/AD0005R on 9145-I716053 -HbA alpha unmutated- (1445 bp)<br> |
- | PCR AD0006F/ca1099R on 9145-I716053 –HbA alpha unmutated-<br> | + | PCR AD0006F/ca1099R on 9145-I716053 –HbA alpha unmutated- (1577 bp)<br> |
Digest both with BsaI/DpnI, and Ligate together Product is 9145-I716073<br> | Digest both with BsaI/DpnI, and Ligate together Product is 9145-I716073<br> |
Latest revision as of 21:52, 26 June 2007
PCR ca1099F/AD0005R on 9145-I716053 -HbA alpha unmutated- (1445 bp)
PCR AD0006F/ca1099R on 9145-I716053 –HbA alpha unmutated- (1577 bp)
Digest both with BsaI/DpnI, and Ligate together Product is 9145-I716073