BerkiGEM2007-AustinConstructionFile24
From 2007.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | Beta portion | + | Beta portion<br> |
| - | pcr CA1154F/G00101 on 9145-I716072 (575 bp) | + | pcr CA1154F/G00101 on 9145-I716072 (575 bp)<br> |
| - | Product is ADtemp1 | + | Product is ADtemp1<br> |
| - | Sub ADtemp1 into pTRC plasmid with (Bgl2/XhoI) | + | Sub ADtemp1 into pTRC plasmid with (Bgl2/XhoI)<br> |
| - | Product is ADtemp2 | + | Product is ADtemp2<br> |
| - | pcr CA1099F/D58 on ADtemp2 (3465 bp) | + | pcr CA1099F/D58 on ADtemp2 (3465 bp)<br> |
| - | Product is ADtemp3 | + | Product is ADtemp3<br> |
| - | _______________________________________________________ | + | _______________________________________________________<br> |
| - | Alpha portion | + | Alpha portion<br> |
| - | Pcr CA1150F/CA1099R on I716073 (1727 bp) | + | Pcr CA1150F/CA1099R on I716073 (1727 bp)<br> |
| - | Product is ADtemp5 | + | Product is ADtemp5<br> |
| - | ____________________________________________________________ | + | ____________________________________________________________<br> |
| - | Digest ADtemp3 (BsaI/BamHI) | + | Digest ADtemp3 (BsaI/BamHI)<br> |
| - | Digest ADtemp5 (BsaI/BglII) | + | Digest ADtemp5 (BsaI/BglII)<br> |
Ligate, product is: I716074 (Best yield) | Ligate, product is: I716074 (Best yield) | ||
| + | <br> | ||
Revision as of 22:49, 30 June 2007
Beta portion
pcr CA1154F/G00101 on 9145-I716072 (575 bp)
Product is ADtemp1
Sub ADtemp1 into pTRC plasmid with (Bgl2/XhoI)
Product is ADtemp2
pcr CA1099F/D58 on ADtemp2 (3465 bp)
Product is ADtemp3
_______________________________________________________
Alpha portion
Pcr CA1150F/CA1099R on I716073 (1727 bp)
Product is ADtemp5
____________________________________________________________
Digest ADtemp3 (BsaI/BamHI)
Digest ADtemp5 (BsaI/BglII)
Ligate, product is: I716074 (Best yield)
