BerkiGEM2007-AustinConstructionFile24

From 2007.igem.org

(Difference between revisions)
Line 4: Line 4:
Sub ADtemp1 into pTRC plasmid with (Bgl2/XhoI)<br>
Sub ADtemp1 into pTRC plasmid with (Bgl2/XhoI)<br>
Product is ADtemp2<br>
Product is ADtemp2<br>
-
pcr CA1099F/D58 on ADtemp2 (3465 bp)<br>
+
pcr CA1099R/D58 on ADtemp2 (3465 bp)<br>
Product is ADtemp3<br>
Product is ADtemp3<br>
_______________________________________________________<br>
_______________________________________________________<br>

Revision as of 02:13, 2 July 2007

Beta portion
pcr CA1154F/G00101 on 9145-I716072 (575 bp)
Product is ADtemp1
Sub ADtemp1 into pTRC plasmid with (Bgl2/XhoI)
Product is ADtemp2
pcr CA1099R/D58 on ADtemp2 (3465 bp)
Product is ADtemp3
_______________________________________________________
Alpha portion
Pcr CA1150F/CA1099R on I716077 ()
Product is ADtemp5
____________________________________________________________
Digest ADtemp3 (BsaI/BamHI)
Digest ADtemp5 (BsaI/BglII)
Ligate, product is: I716074 (Best yield)