BerkiGEM2007-AustinConstructionFile24

From 2007.igem.org

(Difference between revisions)
Line 6: Line 6:
PCR CA1099F/G00101 on Part 9145-I716081 <br>
PCR CA1099F/G00101 on Part 9145-I716081 <br>
-
Digest (Bsa1/BamH1) and ligate with alpha portion. <br>
+
Digest (Bsa1/BamH1) (1447/small, Larger) and ligate with alpha portion. <br>
<br><br>
<br><br>
Product is 9145-I716074 (best yield)
Product is 9145-I716074 (best yield)

Revision as of 18:28, 5 July 2007

Alpha:
PCR CA1150/CA1099R on 9145-I716077 (1727 Bp)
Digest (Bsa1/Bgl2) and ligate with beta portion.

Beta:

PCR CA1099F/G00101 on Part 9145-I716081
Digest (Bsa1/BamH1) (1447/small, Larger) and ligate with alpha portion.


Product is 9145-I716074 (best yield)