BerkiGEM2007-AustinConstructionFile25

From 2007.igem.org

(Difference between revisions)
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Beta portion<br>
Beta portion<br>
pcr CA1154F/G00101 on 9145-I716063 (575 bp)<br>
pcr CA1154F/G00101 on 9145-I716063 (575 bp)<br>
-
Product is ADtemp1<br>
+
Product is ADtemp1b<br>
-
Sub ADtemp1 into pTRC plasmid with (Bgl2/XhoI)<br>
+
Sub ADtemp1b into pTRC plasmid with (Bgl2/XhoI)<br>
-
Product is ADtemp2<br>
+
Product is ADtemp2b<br>
-
pcr CA1099F/D58 on ADtemp2 (3465 bp)<br>
+
pcr CA1099F/D58 on ADtemp2b (3465 bp)<br>
-
Product is ADtemp3<br>
+
Product is ADtemp3b<br>
_______________________________________________________<br>
_______________________________________________________<br>
Alpha portion<br>
Alpha portion<br>
Pcr CA1150F/CA1099R on I716073 (1727 bp)<br>
Pcr CA1150F/CA1099R on I716073 (1727 bp)<br>
-
Product is ADtemp5<br>
+
Product is ADtemp5b<br>
____________________________________________________________<br>
____________________________________________________________<br>
-
Digest ADtemp3 (BsaI/BamHI)<br>
+
Digest ADtemp3b (BsaI/BamHI)<br>
-
Digest ADtemp5 (BsaI/BglII)<br>
+
Digest ADtemp5b (BsaI/BglII)<br>
Ligate, product is: I716075 (Best all around)
Ligate, product is: I716075 (Best all around)
<br>
<br>

Revision as of 22:51, 30 June 2007

Beta portion
pcr CA1154F/G00101 on 9145-I716063 (575 bp)
Product is ADtemp1b
Sub ADtemp1b into pTRC plasmid with (Bgl2/XhoI)
Product is ADtemp2b
pcr CA1099F/D58 on ADtemp2b (3465 bp)
Product is ADtemp3b
_______________________________________________________
Alpha portion
Pcr CA1150F/CA1099R on I716073 (1727 bp)
Product is ADtemp5b
____________________________________________________________
Digest ADtemp3b (BsaI/BamHI)
Digest ADtemp5b (BsaI/BglII)
Ligate, product is: I716075 (Best all around)