BerkiGEM2007-AustinConstructionFile26

From 2007.igem.org

(Difference between revisions)
Line 13: Line 13:
Digest ADtemp3c (BsaI/BamHI)<br>
Digest ADtemp3c (BsaI/BamHI)<br>
Digest ADtemp5c (BsaI/BglII)<br>
Digest ADtemp5c (BsaI/BglII)<br>
-
Ligate, product is: I716075 (Second best all around)
+
Ligate, product is: I716076 (Second best all around)
<br>
<br>

Revision as of 22:53, 30 June 2007

Beta portion
pcr CA1154F/G00101 on 9145-I716059 (575 bp)
Product is ADtemp1c
Sub ADtemp1c into pTRC plasmid with (Bgl2/XhoI)
Product is ADtemp2c
pcr CA1099F/D58 on ADtemp2c (3465 bp)
Product is ADtemp3c
_______________________________________________________
Alpha portion
Pcr CA1150F/CA1099R on I716062 (1727 bp)
Product is ADtemp5c
____________________________________________________________
Digest ADtemp3c (BsaI/BamHI)
Digest ADtemp5c (BsaI/BglII)
Ligate, product is: I716076 (Second best all around)