BerkiGEM2007-AustinConstructionFile26

From 2007.igem.org

(Difference between revisions)
 
(5 intermediate revisions not shown)
Line 1: Line 1:
-
Beta portion<br>
+
Alpha: <br>
-
pcr CA1154F/G00101 on 9145-I716059 (575 bp)<br>
+
PCR CA1150/CA1099R on 9145-I716078 (1727 Bp)<br>
-
Product is ADtemp1c<br>
+
Digest (Bsa1/Bgl2)(~2000/Small, Larger) and ligate with beta portion.<br> <br>
-
Sub ADtemp1c into pTRC plasmid with (Bgl2/XhoI)<br>
+
 
-
Product is ADtemp2c<br>
+
Beta: <br>
-
pcr CA1099F/D58 on ADtemp2c (3465 bp)<br>
+
 
-
Product is ADtemp3c<br>
+
PCR CA1099F/G00101 on Part 9145-I716081 <br>
-
_______________________________________________________<br>
+
Digest (Bsa1/BamH1)(1447/Small, Larger) and ligate with alpha portion. <br>
-
Alpha portion<br>
+
<br><br>
-
Pcr CA1150F/CA1099R on I716062 (1727 bp)<br>
+
Product is 9145-I716076 (Second best all around)
-
Product is ADtemp5c<br>
+
-
____________________________________________________________<br>
+
-
Digest ADtemp3c (BsaI/BamHI)<br>
+
-
Digest ADtemp5c (BsaI/BglII)<br>
+
-
Ligate, product is: I716075 (Second best all around)
+
-
<br>
+

Latest revision as of 18:31, 5 July 2007

Alpha:
PCR CA1150/CA1099R on 9145-I716078 (1727 Bp)
Digest (Bsa1/Bgl2)(~2000/Small, Larger) and ligate with beta portion.

Beta:

PCR CA1099F/G00101 on Part 9145-I716081
Digest (Bsa1/BamH1)(1447/Small, Larger) and ligate with alpha portion.


Product is 9145-I716076 (Second best all around)