BerkiGEM2007-AustinConstructionFile26

From 2007.igem.org

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Beta portion<br>
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Alpha: <br>
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pcr CA1154F/G00101 on 9145-I716059 (575 bp)<br>
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PCR CA1150/CA1099R on 9145-I716078 (1727 Bp)<br>
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Product is ADtemp1c<br>
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Digest (Bsa1/Bgl2)(~2000/Small, Larger) and ligate with beta portion.<br> <br>
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Sub ADtemp1c into pTRC plasmid with (Bgl2/XhoI)<br>
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Product is ADtemp2c<br>
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Beta: <br>
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pcr CA1099F/D58 on ADtemp2c (3465 bp)<br>
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Product is ADtemp3c<br>
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PCR CA1099F/G00101 on Part 9145-I716081 <br>
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_______________________________________________________<br>
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Digest (Bsa1/BamH1)(1447/Small, Larger) and ligate with alpha portion. <br>
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Alpha portion<br>
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<br><br>
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Pcr CA1150F/CA1099R on I716062 (1727 bp)<br>
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Product is 9145-I716076 (Second best all around)
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Product is ADtemp5c<br>
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____________________________________________________________<br>
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Digest ADtemp3c (BsaI/BamHI)<br>
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Digest ADtemp5c (BsaI/BglII)<br>
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Ligate, product is: I716076 (Second best all around)
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<br>
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Latest revision as of 18:31, 5 July 2007

Alpha:
PCR CA1150/CA1099R on 9145-I716078 (1727 Bp)
Digest (Bsa1/Bgl2)(~2000/Small, Larger) and ligate with beta portion.

Beta:

PCR CA1099F/G00101 on Part 9145-I716081
Digest (Bsa1/BamH1)(1447/Small, Larger) and ligate with alpha portion.


Product is 9145-I716076 (Second best all around)