BerkiGEM2007-AustinConstructionFile37

From 2007.igem.org

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PCR AD0010F/AD0010R on I716054 (~480 bp)
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PCR AD0010F/AD0010R on I716054 (462 bp)
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Digest with EcoR1/Nco1
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Digest with EcoR1/Nco1 (446/11/5) Largest
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Digest petDUET (EcoR1/Nco1, ?/?, Largest)
+
Digest petDUET (EcoR1/Nco1, 5377/43, Larger)
Ligation product is I716087
Ligation product is I716087

Revision as of 05:51, 27 July 2007

PCR AD0010F/AD0010R on I716054 (462 bp) Digest with EcoR1/Nco1 (446/11/5) Largest

Digest petDUET (EcoR1/Nco1, 5377/43, Larger)

Ligation product is I716087