BerkiGEM2007-AustinConstructionFile37

From 2007.igem.org

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PCR AD0010F/AD0010R on I716054 (462 bp)<br>
PCR AD0010F/AD0010R on I716054 (462 bp)<br>
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Digest with EcoR1/Nco1 (446/11/5) Largest<br>
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Digest PCR product (EcoR1/Nco1/DpnI, 446/11/5, Largest)<br>
Digest petDUET (EcoR1/Nco1, 5377/43, Larger)<br>
Digest petDUET (EcoR1/Nco1, 5377/43, Larger)<br>
Ligation product is I716087<br>
Ligation product is I716087<br>

Latest revision as of 01:07, 30 July 2007

PCR AD0010F/AD0010R on I716054 (462 bp)
Digest PCR product (EcoR1/Nco1/DpnI, 446/11/5, Largest)

Digest petDUET (EcoR1/Nco1, 5377/43, Larger)

Ligation product is I716087