BerkiGEM2007-AustinConstructionFile38

From 2007.igem.org

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Digest I716011 (EcoR1/BamH1, 2949/1231, Smaller)<br>
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PCR AD0010F/AD0010R on I716072 (462 bp)<br>
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Digest I716074 (EcoR1/Bgl2, 3541/9, Larger)<br>
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Digest PCR product (EcoR1/Nco1/DpnI, 446/11/5, Largest)<br>
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Ligated Product is I716088<br>
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Digest petDUET (EcoR1/Nco1, 5377/43, Larger)<br>
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Ligation product is I716088<br>

Latest revision as of 01:07, 30 July 2007

PCR AD0010F/AD0010R on I716072 (462 bp)
Digest PCR product (EcoR1/Nco1/DpnI, 446/11/5, Largest)

Digest petDUET (EcoR1/Nco1, 5377/43, Larger)

Ligation product is I716088