BerkiGEM2007-AustinConstructionFile39

From 2007.igem.org

(Difference between revisions)
 
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PCR AD0010F/AD0010R on I716063 (462 bp)<br>
PCR AD0010F/AD0010R on I716063 (462 bp)<br>
-
Digest PCR product (EcoR1/Nco1, 446/11/5, Largest)<br>
+
Digest PCR product (EcoR1/Nco1/DpnI, 446/11/5, Largest)<br>
Digest petDUET (EcoR1/Nco1, 5377/43, Larger)<br>
Digest petDUET (EcoR1/Nco1, 5377/43, Larger)<br>
Ligation product is I716089<br>
Ligation product is I716089<br>

Latest revision as of 01:07, 30 July 2007

PCR AD0010F/AD0010R on I716063 (462 bp)
Digest PCR product (EcoR1/Nco1/DpnI, 446/11/5, Largest)

Digest petDUET (EcoR1/Nco1, 5377/43, Larger)

Ligation product is I716089