BerkiGEM2007-AustinConstructionFile54

From 2007.igem.org

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Quickchange AD0015F/R to get rid of XhoI site. <br>
Quickchange AD0015F/R to get rid of XhoI site. <br>
<br>
<br>
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PCR AD0016F/R on modified I716091.
+
PCR AD0016F/R on modified I716091.<br>
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Digest Bgl2/Xho1  and insert into 9145 plasmid.
+
Digest Bgl2/Xho1  and insert into 9145 plasmid.<br>
 +
Product is I716354<br>

Latest revision as of 01:50, 9 August 2007

Pre-biobricking modifications to I716091.
Quickchange AD0013F/R to get rid of EcoR1 site.
Quickchange AD0014/F/R to get rid of BglII site.
Quickchange AD0015F/R to get rid of XhoI site.

PCR AD0016F/R on modified I716091.
Digest Bgl2/Xho1 and insert into 9145 plasmid.
Product is I716354