BerkiGEM2007-AustinConstructionFile55

From 2007.igem.org

(Difference between revisions)

Revision as of 01:50, 9 August 2007

Pre-biobricking modifications to I716092.
Quickchange AD0013F/R to get rid of EcoR1 site.
Quickchange AD0014/F/R to get rid of BglII site.
Quickchange AD0015F/R to get rid of XhoI site.

PCR AD0016F/R on modified I716092. Digest Bgl2/Xho1 and insert into 9145 plasmid. Product is I716355