BerkiGEM2007-AustinConstructionFile62

From 2007.igem.org

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Digest I716269 (BglII/XhoI/ScaI, 2835/1504/559, largest) <br>
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Digest I716269 (BglII/XhoI, 2835/2063, largest) <br>
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Digest I716354 (BamHI/Xho, 3441/9, Larger) <br>
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Digest I716354 (BamHI/XhoI, 3441/9, Larger) <br>
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Ligation product is I716362<br>
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1,2,3 method ligation product is I716362<br>
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<br><br>
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Dbbs Method:
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PCR 1099R/ca998 on I716269 (4032bp, BglII, BsaI, DpnI)<br>
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PCR 1099F/g00101 on I716354 (2479bp, BamHI, BsaI, DpnI)<br>
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Ligation product is I716362 (6282 bp)<br>
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Latest revision as of 04:54, 20 September 2007

Digest I716269 (BglII/XhoI, 2835/2063, largest)
Digest I716354 (BamHI/XhoI, 3441/9, Larger)
1,2,3 method ligation product is I716362

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