BerkiGEM2007-AustinConstructionFile62
From 2007.igem.org
(Difference between revisions)
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Digest I716354 (BamHI/Xho, 3441/9, Larger) <br> | Digest I716354 (BamHI/Xho, 3441/9, Larger) <br> | ||
Ligation product is I716362<br> | Ligation product is I716362<br> | ||
+ | |||
+ | |||
+ | <br><br> | ||
+ | Dbbs Method: | ||
+ | PCR 1099R/ca998 on I716269 (4032bp, BglII, BsaI, DpnI)<br> | ||
+ | PCR 1099F/g00101 on I716354 (2479bp, BamHI, BsaI, DpnI)<br> | ||
+ | Ligation product is I716362 (6282 bp)<br> |
Revision as of 21:19, 10 September 2007
Digest I716269 (BglII/XhoI/ScaI, 2835/1504/559, largest)
Digest I716354 (BamHI/Xho, 3441/9, Larger)
Ligation product is I716362
Dbbs Method:
PCR 1099R/ca998 on I716269 (4032bp, BglII, BsaI, DpnI)
PCR 1099F/g00101 on I716354 (2479bp, BamHI, BsaI, DpnI)
Ligation product is I716362 (6282 bp)