BerkiGEM2007-AustinConstructionFile62
From 2007.igem.org
Digest I716269 (BglII/XhoI/ScaI, 2835/1504/559, largest)
Digest I716354 (BamHI/Xho, 3441/9, Larger)
Ligation product is I716362
Dbbs Method:
PCR 1099R/ca998 on I716269 (4032bp, BglII, BsaI, DpnI)
PCR 1099F/g00101 on I716354 (2479bp, BamHI, BsaI, DpnI)
Ligation product is I716362 (6282 bp)