BerkiGEM2007-DavidConstructionFile103

From 2007.igem.org

(Difference between revisions)
m
m
Line 8: Line 8:
Product G is pBca9145-I716104-T7rnap-G
Product G is pBca9145-I716104-T7rnap-G
---------------------------------------------------
---------------------------------------------------
-
dt005-T7rnap-FA  Forward Cloning of T7rnap ATG  ccaaaAGATCTatgaacacgattaacatcgc
+
dt005-T7rnap-FA  Forward Cloning of T7rnap ATG Start   ccaaaAGATCTatgaacacgattaacatcgc
-
dt006-T7rnap-FG  Forward Cloning of T7rnap GTG  ccaaaAGATCTgtgaacacgattaacatcgc
+
dt006-T7rnap-FG  Forward Cloning of T7rnap GTG Start   ccaaaAGATCTgtgaacacgattaacatcgc
-
dt007-T7rnap-R  Reverse Cloning of T7rnap       gctagCTCGAGttaGGATCCttacgcgaacgcgaagtccg
+
dt007-T7rnap-R  Reverse Cloning of T7rnap       gctagCTCGAGttaGGATCCttacgcgaacgcgaagtccg
</pre>
</pre>

Revision as of 06:39, 5 June 2007

Biobrickificreation of T7rnap I716103, I716104 (T7 RNA Polymerase: ATG, GTG)
---------------------------------------------------
PCR T7rnap-FA and T7rnap-R on pBAC-T7621	(2683 bp, BglII/XhoI, Product = A)
PCR T7rnap-FG and T7rnap-R on pBAC-T7621	(2683 bp, BglII/XhoI, Product = G)
Sub both into pBca9145-Bca1144			(BglII/XhoI, 2063+910, L)
Product A is pBca9145-I716103-T7rnap-A
Product G is pBca9145-I716104-T7rnap-G
---------------------------------------------------
dt005-T7rnap-FA  Forward Cloning of T7rnap ATG Start   ccaaaAGATCTatgaacacgattaacatcgc
dt006-T7rnap-FG  Forward Cloning of T7rnap GTG Start   ccaaaAGATCTgtgaacacgattaacatcgc
dt007-T7rnap-R   Reverse Cloning of T7rnap	       gctagCTCGAGttaGGATCCttacgcgaacgcgaagtccg