BerkiGEM2007-DavidConstructionFile105
From 2007.igem.org
(Difference between revisions)
(New Construction File) |
m |
||
| (One intermediate revision not shown) | |||
| Line 1: | Line 1: | ||
| + | <pre> | ||
Biobrickificreation of T7 Promoters I716105, I716106, I716107 (Wild Type, Mutant D, Mutant E) | Biobrickificreation of T7 Promoters I716105, I716106, I716107 (Wild Type, Mutant D, Mutant E) | ||
--------------------------------------------------- | --------------------------------------------------- | ||
| Line 6: | Line 7: | ||
Sub each separately into pBca9145-Bca1144 (EcoRI/BamHI, 2063+910, L) | Sub each separately into pBca9145-Bca1144 (EcoRI/BamHI, 2063+910, L) | ||
Product A is pBca9145-I716105-PT7-wt | Product A is pBca9145-I716105-PT7-wt | ||
| - | Product D is pBca9145- | + | Product D is pBca9145-I716106-PT7-D |
| - | Product E is pBca9145- | + | Product E is pBca9145-I716107-PT7-E |
--------------------------------------------------- | --------------------------------------------------- | ||
dt008-PT7-wt T7 Promoter (Wild Type) GtttGGATCCaccTCTCCCTATAGTGAGTCGTATTAtacAGATCTcatGAATTCcagaa | dt008-PT7-wt T7 Promoter (Wild Type) GtttGGATCCaccTCTCCCTATAGTGAGTCGTATTAtacAGATCTcatGAATTCcagaa | ||
dt009-PT7-D T7 Promoter (Mutant "D") GtttGGATCCaccTCTCCCTTTAGTGAGTCGTATTAtacAGATCTcatGAATTCcagaa | dt009-PT7-D T7 Promoter (Mutant "D") GtttGGATCCaccTCTCCCTTTAGTGAGTCGTATTAtacAGATCTcatGAATTCcagaa | ||
dt010-PT7-E T7 Promoter (Mutant "E") GtttGGATCCaccTGCTTCTATAGTGAGTCGTATTAtacAGATCTcatGAATTCcagaa | dt010-PT7-E T7 Promoter (Mutant "E") GtttGGATCCaccTGCTTCTATAGTGAGTCGTATTAtacAGATCTcatGAATTCcagaa | ||
| + | </pre> | ||
Latest revision as of 04:49, 8 June 2007
Biobrickificreation of T7 Promoters I716105, I716106, I716107 (Wild Type, Mutant D, Mutant E) --------------------------------------------------- PCR ca1090F and dt008-PT7-wt (62 bp, EcoRI/BamHI, Product = A) PCR ca1090F and dt009-PT7-D (62 bp, EcoRI/BamHI, Product = D) PCR ca1090F and dt010-PT7-E (62 bp, EcoRI/BamHI, Product = E) Sub each separately into pBca9145-Bca1144 (EcoRI/BamHI, 2063+910, L) Product A is pBca9145-I716105-PT7-wt Product D is pBca9145-I716106-PT7-D Product E is pBca9145-I716107-PT7-E --------------------------------------------------- dt008-PT7-wt T7 Promoter (Wild Type) GtttGGATCCaccTCTCCCTATAGTGAGTCGTATTAtacAGATCTcatGAATTCcagaa dt009-PT7-D T7 Promoter (Mutant "D") GtttGGATCCaccTCTCCCTTTAGTGAGTCGTATTAtacAGATCTcatGAATTCcagaa dt010-PT7-E T7 Promoter (Mutant "E") GtttGGATCCaccTGCTTCTATAGTGAGTCGTATTAtacAGATCTcatGAATTCcagaa
