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Revision as of 21:30, 28 June 2007 (view source) Hcole (Talk | contribs) ← Older edit		Revision as of 18:30, 13 July 2007 (view source) Hcole (Talk | contribs) Newer edit →
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		+	Grow BBAI716316 in Lefty strain. Grow BCA1092 in Righty Strain.
-	PCR HNX-F and HNX-R on H-Nox  (567bp, BglII/XhoI)
-	Sub into pBca1144              ( BglII/XhoI, 2054+910  L)
-	Product is pBca1144-H-NOX
-	----------------------------------------------------------
-	HNX-F Forward primer anneals to BglII ctagtAGATCTATGAAGGGGACAATCGTCGG
-	HNX-R Reverse primer anneals to XhoI cgtgaCTCGAGgaatgGGATCCTTAATTTTTCTTATACTCA	+	Digest BBA_I716316 with EcoRI and BamHI. Digest BCA1092 with EcoRI BglII.
		+
		+	Ligate together.

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Revision as of 18:30, 13 July 2007

Grow BBAI716316 in Lefty strain. Grow BCA1092 in Righty Strain.

Digest BBA_I716316 with EcoRI and BamHI. Digest BCA1092 with EcoRI BglII.

Ligate together.