BerkiGEM2007-KristinConstructionFile12

From 2007.igem.org

(Difference between revisions)
 
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Composite part of RBS (pBca1101-1106A) + hemA from CFT073
Composite part of RBS (pBca1101-1106A) + hemA from CFT073
-
Digest pBca1101-1106A (BamH1/XhoI, 2132+850, L)
+
Digest pBca1101-1106A         (BamH1/XhoI, 2132+850, L)
Sub into pBca9145-I716152 (Bg1II/XhoI, 2063+1272, S)
Sub into pBca9145-I716152 (Bg1II/XhoI, 2063+1272, S)
Product is pBca9145-I716162
Product is pBca9145-I716162
</pre>
</pre>

Latest revision as of 17:09, 17 July 2007

Composite part of RBS (pBca1101-1106A) + hemA from CFT073

Digest pBca1101-1106A		        (BamH1/XhoI, 2132+850, L)
Sub into pBca9145-I716152		(Bg1II/XhoI, 2063+1272, S)
Product is pBca9145-I716162