BerkiGEM2007-KristinConstructionFile6

From 2007.igem.org

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<pre>
Composite part of RBS (pBca1101-I716051-kan cassette) + hemA from R.capsulatus
Composite part of RBS (pBca1101-I716051-kan cassette) + hemA from R.capsulatus
Digest pBca1101-I716051         (EcoR1/BamH1, 2904+1065, S)
Digest pBca1101-I716051         (EcoR1/BamH1, 2904+1065, S)
Sub into pBca9145-I716151 (EcoR1/Bg1II, 3275+9, L)
Sub into pBca9145-I716151 (EcoR1/Bg1II, 3275+9, L)
Product is pBca9145-I716156
Product is pBca9145-I716156
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</pre>

Revision as of 17:05, 17 July 2007

Composite part of RBS (pBca1101-I716051-kan cassette) + hemA from R.capsulatus
Digest pBca1101-I716051		        (EcoR1/BamH1, 2904+1065, S)
Sub into pBca9145-I716151		(EcoR1/Bg1II, 3275+9, L)
Product is pBca9145-I716156