BerkiGEM2007-SamanthaConstructionFile16
From 2007.igem.org
(Difference between revisions)
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Digest pBca9145-I716214 (BamHI/AlwnI/BglI, 1195+596+553bp, Large) <br> | Digest pBca9145-I716214 (BamHI/AlwnI/BglI, 1195+596+553bp, Large) <br> | ||
Digest pBca9145-Bca1146 (BglII/BglI, 1824+923bp, Large) <br> | Digest pBca9145-Bca1146 (BglII/BglI, 1824+923bp, Large) <br> | ||
| - | Product is pBca9145- | + | Product is pBca9145-I716422. <br> |
Digest pBca9145-I716215 (BamHI/AlwnI/BglI, 1166+596+553bp, Large) <br> | Digest pBca9145-I716215 (BamHI/AlwnI/BglI, 1166+596+553bp, Large) <br> | ||
Digest pBca9145-Bca1146 (BglII/BglI, 1824+923bp, Large) <br> | Digest pBca9145-Bca1146 (BglII/BglI, 1824+923bp, Large) <br> | ||
| - | Product is pBca9145- | + | Product is pBca9145-I716423. <br> |
Latest revision as of 16:55, 10 July 2007
[rbs][ATG][lox][term][lox][RFP]
2 Different terminators (1124 and 1092 - will probably use 1092 in the end)
using Bca1090 rbs site
note - RFP still has it's start codon unlike the toxins
Digest pBca9145-I716214 (BamHI/AlwnI/BglI, 1195+596+553bp, Large)
Digest pBca9145-Bca1146 (BglII/BglI, 1824+923bp, Large)
Product is pBca9145-I716422.
Digest pBca9145-I716215 (BamHI/AlwnI/BglI, 1166+596+553bp, Large)
Digest pBca9145-Bca1146 (BglII/BglI, 1824+923bp, Large)
Product is pBca9145-I716423.
