BerkiGEM2007-SamanthaConstructionFile19

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[rbs][Cre]
2 Different rbs variants (1106A and 1090)
GTG Cre



Digest pBca1101-Bca1106A (BamHI/BglI, 1990+992bp, Small)
Digest pBca9145-I716212 (BglII/BglI, 2187+923bp, Large)
Product is pBca1101-I716436.


Digest pBca1101-Bca1106B (BamHI/BglI, 1149+942bp, Small)
Digest pBca9145-I716212 (BglII/BglI, 2187+923bp, Large)
Product is pBca9145-I716437.