BerkiGEM2007-SamanthaConstructionFile19

From 2007.igem.org

[rbs][Cre]
2 Different rbs variants (1106A and 1090)
GTG Cre



Digest pBca1101-Bca1106A (BamHI/BglI, 1990+992bp, Small)
Digest pBca9145-I716212 (BglII/BglI, 2187+923bp, Large)
Product is pBca1101-I716436.


Digest pBca1101-Bca1106B (BamHI/BglI, 1149+942bp, Small)
Digest pBca9145-I716212 (BglII/BglI, 2187+923bp, Large)
Product is pBca9145-I716437.