BerkiGEM2007-SamanthaConstructionFile3

From 2007.igem.org

(Difference between revisions)
 
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'''[lox][term][lox]''' <br>
'''[lox][term][lox]''' <br>
-
'''2 Different terminators (1124 and 1092 (will probably use 1092 in the end))''' <br>
+
'''2 Different terminators (1124 and 1092 - will probably use 1092 in the end)''' <br>
----
----
-
Digest pBca9145-I716205 (BamHI/AlwnI, 1559+553bp, Large) <br>
+
Digest pBca9145-I716205 (BamHI/AlwnI, 1723+553bp, Large) <br>
Digest pBca9145-Bca1114 (BglII/AlwnI, 1519+593bp, Small) <br>
Digest pBca9145-Bca1114 (BglII/AlwnI, 1519+593bp, Small) <br>
Product is pBca9145-I716207. <br>
Product is pBca9145-I716207. <br>
-
Digest pBca9145-I716205 (BamHI/AlwnI, 1559+553bp, Large) <br>
+
Digest pBca9145-I716206 (BamHI/AlwnI, 1694+553bp, Large) <br>
Digest pBca9145-Bca1114 (BglII/AlwnI, 1519+593bp, Small) <br>
Digest pBca9145-Bca1114 (BglII/AlwnI, 1519+593bp, Small) <br>
Product is pBca9145-I716208. <br>
Product is pBca9145-I716208. <br>

Latest revision as of 18:56, 5 June 2007

[lox][term][lox]
2 Different terminators (1124 and 1092 - will probably use 1092 in the end)



Digest pBca9145-I716205 (BamHI/AlwnI, 1723+553bp, Large)
Digest pBca9145-Bca1114 (BglII/AlwnI, 1519+593bp, Small)
Product is pBca9145-I716207.


Digest pBca9145-I716206 (BamHI/AlwnI, 1694+553bp, Large)
Digest pBca9145-Bca1114 (BglII/AlwnI, 1519+593bp, Small)
Product is pBca9145-I716208.