BerkiGEM2007-SamanthaConstructionFile4

From 2007.igem.org

(Difference between revisions)
 
(One intermediate revision not shown)
Line 4: Line 4:
PCR sil02F and sil02R on colE2 colicin region          (1758bp, BglII/XhoI) <br>
PCR sil02F and sil02R on colE2 colicin region          (1758bp, BglII/XhoI) <br>
Sub into pBca9145-Bca1089                              (BglII/XhoI, 2063+696, L) <br>
Sub into pBca9145-Bca1089                              (BglII/XhoI, 2063+696, L) <br>
-
Product is pBca9145-Bsl2001
+
Product is pBca9145-I716209
----
----

Latest revision as of 18:23, 5 June 2007

Biobricking of ceaB without start codon


PCR sil02F and sil02R on colE2 colicin region (1758bp, BglII/XhoI)
Sub into pBca9145-Bca1089 (BglII/XhoI, 2063+696, L)
Product is pBca9145-I716209


sil02F Forward BglII biobrick of ceaB ccaaaAGATCTagcggtggcgatggacgcgg
sil02R Reverse XholI/BamHI biobrick of ceaB gctagCTCGAGttaGGATCCttacttaccccgatgaatatc