BerkiGEM2007-SamanthaConstructionFile5

From 2007.igem.org

(Difference between revisions)
 
(One intermediate revision not shown)
Line 2: Line 2:
-
PCR sil04F and sil04R on BamHI methylase/endonuclease region. (657 bp, BglII/XhoI)
+
PCR sil04F and sil04R on BamHI methylase/endonuclease region. (657 bp, BglII/XhoI) <br>
-
Sub into pBca9145-Bca1089                                      (BglII/XhoI,2063+696, L)
+
Sub into pBca9145-Bca1089                                      (BglII/XhoI,2063+696, L) <br>
-
Product is pBca9145-I716209
+
Product is pBca9145-I716210 <br>
----------------------------
----------------------------
-
sil04F  Forward BglII biobrick of BamHI           ccaaaAGATCTgaagtagaaaaagagtttattactga
+
sil04F  Forward BglII biobrick of BamHI     <br>      ccaaaAGATCTgaagtagaaaaagagtttattactga <br>
-
sil04R  Reverse XholI/BamHI biobrick of BamHI     gctagCTCGAGttaGGATCCctatttgttttcaactttatc
+
sil04R  Reverse XholI/BamHI biobrick of BamHI <br>    gctagCTCGAGttaGGATCCctatttgttttcaactttatc <br>

Latest revision as of 18:23, 5 June 2007

Biobricking of BamHI endonuclease without start codon


PCR sil04F and sil04R on BamHI methylase/endonuclease region. (657 bp, BglII/XhoI)
Sub into pBca9145-Bca1089 (BglII/XhoI,2063+696, L)
Product is pBca9145-I716210


sil04F Forward BglII biobrick of BamHI
ccaaaAGATCTgaagtagaaaaagagtttattactga
sil04R Reverse XholI/BamHI biobrick of BamHI
gctagCTCGAGttaGGATCCctatttgttttcaactttatc