BerkiGEM2007-SamanthaConstructionFile6
From 2007.igem.org
(Difference between revisions)
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| - | PCR sil03F and sil03R on barnase region (348 bp, BglII/XhoI) | + | PCR sil03F and sil03R on barnase region (348 bp, BglII/XhoI) <br> |
| - | Sub into pBca9145-Bca1089 (BglII/XhoI, 2063+696, L) | + | Sub into pBca9145-Bca1089 (BglII/XhoI, 2063+696, L) <br> |
| - | Product is pBca9145- | + | Product is pBca9145-I716210 <br> |
---------------------------- | ---------------------------- | ||
| - | sil03F Forward BglII biobrick of barnase ccaaaAGATCTgcacaggttatcaacacgtttg | + | sil03F Forward BglII biobrick of barnase ccaaaAGATCTgcacaggttatcaacacgtttg <br> |
| - | sil03R Reverse XholI/BamHI biobrick of barnase gctagCTCGAGttaGGATCCttatctgatttttgtaaagg | + | sil03R Reverse XholI/BamHI biobrick of barnase gctagCTCGAGttaGGATCCttatctgatttttgtaaagg <br> |
Revision as of 22:56, 4 June 2007
Biobricking of barnase active codon
PCR sil03F and sil03R on barnase region (348 bp, BglII/XhoI)
Sub into pBca9145-Bca1089 (BglII/XhoI, 2063+696, L)
Product is pBca9145-I716210
sil03F Forward BglII biobrick of barnase ccaaaAGATCTgcacaggttatcaacacgtttg
sil03R Reverse XholI/BamHI biobrick of barnase gctagCTCGAGttaGGATCCttatctgatttttgtaaagg
