BerkiGEM2007-SamanthaConstructionFile7

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Construction of Cre biobrick with weaker start codon
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'''Construction of Cre biobrick with weaker start codon'''
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PCR sil05 and G00101 on pBca9145-Bca1117              (PCR product is 1147bp, BglII/XhoI 1047 bp) <br>
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Sub into pBca9145-Bca1089                              (BglII/XhoI, 2063+696, L) <br>
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Product is pBca9145-I716212 (GTG start)<br>
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PCR sil06 and G00101 on pBca9145-Bca1117                (PCR product is 1147bp, BglII/XhoI 1047 bp) <br>
 +
Sub into pBca9145-Bca1089                              (BglII/XhoI, 2063+696, L) <br>
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Product is pBca9145-I716213 (TTG start)<br>
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sil05  Forward BglII Cre with GTG start      ctagcAGATCTgtgtccaatttactgaccgtac<br>
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sil06  Forward BglII Cre with TTG start      ctagcAGATCTttgtccaatttactgaccgtac<br>
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G00101  Reverse Sequencing of pSB1A* plasmids  attaccgcctttgagtgagc

Latest revision as of 22:56, 5 June 2007

Construction of Cre biobrick with weaker start codon


PCR sil05 and G00101 on pBca9145-Bca1117 (PCR product is 1147bp, BglII/XhoI 1047 bp)
Sub into pBca9145-Bca1089 (BglII/XhoI, 2063+696, L)
Product is pBca9145-I716212 (GTG start)


PCR sil06 and G00101 on pBca9145-Bca1117 (PCR product is 1147bp, BglII/XhoI 1047 bp)
Sub into pBca9145-Bca1089 (BglII/XhoI, 2063+696, L)
Product is pBca9145-I716213 (TTG start)


sil05 Forward BglII Cre with GTG start ctagcAGATCTgtgtccaatttactgaccgtac
sil06 Forward BglII Cre with TTG start ctagcAGATCTttgtccaatttactgaccgtac
G00101 Reverse Sequencing of pSB1A* plasmids attaccgcctttgagtgagc