BerkiGEM2007-SamanthaConstructionFile7

From 2007.igem.org

(Difference between revisions)
 
(One intermediate revision not shown)
Line 5: Line 5:
PCR sil05 and G00101 on pBca9145-Bca1117              (PCR product is 1147bp, BglII/XhoI 1047 bp) <br>
PCR sil05 and G00101 on pBca9145-Bca1117              (PCR product is 1147bp, BglII/XhoI 1047 bp) <br>
Sub into pBca9145-Bca1089                              (BglII/XhoI, 2063+696, L) <br>
Sub into pBca9145-Bca1089                              (BglII/XhoI, 2063+696, L) <br>
-
Product is pBca9145-I716211 (GTG start)<br>
+
Product is pBca9145-I716212 (GTG start)<br>
-
PCR sil05 and G00101 on pBca9145-Bca1117                (PCR product is 1147bp, BglII/XhoI 1047 bp) <br>
+
PCR sil06 and G00101 on pBca9145-Bca1117                (PCR product is 1147bp, BglII/XhoI 1047 bp) <br>
Sub into pBca9145-Bca1089                              (BglII/XhoI, 2063+696, L) <br>
Sub into pBca9145-Bca1089                              (BglII/XhoI, 2063+696, L) <br>
-
Product is pBca9145-I716212 (TTG start)<br>
+
Product is pBca9145-I716213 (TTG start)<br>
----------------------------
----------------------------
sil05  Forward BglII Cre with GTG start      ctagcAGATCTgtgtccaatttactgaccgtac<br>
sil05  Forward BglII Cre with GTG start      ctagcAGATCTgtgtccaatttactgaccgtac<br>
sil06  Forward BglII Cre with TTG start      ctagcAGATCTttgtccaatttactgaccgtac<br>
sil06  Forward BglII Cre with TTG start      ctagcAGATCTttgtccaatttactgaccgtac<br>
G00101  Reverse Sequencing of pSB1A* plasmids  attaccgcctttgagtgagc
G00101  Reverse Sequencing of pSB1A* plasmids  attaccgcctttgagtgagc

Latest revision as of 22:56, 5 June 2007

Construction of Cre biobrick with weaker start codon


PCR sil05 and G00101 on pBca9145-Bca1117 (PCR product is 1147bp, BglII/XhoI 1047 bp)
Sub into pBca9145-Bca1089 (BglII/XhoI, 2063+696, L)
Product is pBca9145-I716212 (GTG start)


PCR sil06 and G00101 on pBca9145-Bca1117 (PCR product is 1147bp, BglII/XhoI 1047 bp)
Sub into pBca9145-Bca1089 (BglII/XhoI, 2063+696, L)
Product is pBca9145-I716213 (TTG start)


sil05 Forward BglII Cre with GTG start ctagcAGATCTgtgtccaatttactgaccgtac
sil06 Forward BglII Cre with TTG start ctagcAGATCTttgtccaatttactgaccgtac
G00101 Reverse Sequencing of pSB1A* plasmids attaccgcctttgagtgagc