BerkiGEM2007-SamanthaConstructionFile8
From 2007.igem.org
| Line 1: | Line 1: | ||
| - | NOT DONE YET...found an ALWNI site in Cre | + | NOT DONE YET...found an ALWNI site in Cre, use BglI instead |
'''[rbs][ATG]''' <br> | '''[rbs][ATG]''' <br> | ||
| Line 8: | Line 8: | ||
| - | Digest pBca1101-Bca1106A (BamHI/ | + | Digest pBca1101-Bca1106A (BamHI/BglI, 1588+850+544bp, Large) <br> |
| - | Digest pBca9145-I716212 (BglII/ | + | Digest pBca9145-I716212 (BglII/BglI, 2187+923bp, Small) <br> |
Product is pBca9145-I716214. <br> | Product is pBca9145-I716214. <br> | ||
| - | Digest pBca1101-Bca1106B (BamHI/ | + | Digest pBca1101-Bca1106B (BamHI/BglI, 1588+850+544bp, Large) <br> |
| - | Digest pBca9145-I716212 (BglII/ | + | Digest pBca9145-I716212 (BglII/BglI, 2187+923bp, Small) <br> |
Product is pBca9145-I716215. <br> | Product is pBca9145-I716215. <br> | ||
| - | Digest pBca1101-Bca1128A (BamHI/ | + | Digest pBca1101-Bca1128A (BamHI/BglI, 1606+850+544bp, Large) <br> |
| - | Digest pBca9145-I716212 (BglII/ | + | Digest pBca9145-I716212 (BglII/BglI, 2187+923bp, Small) <br> |
Product is pBca9145-I716216. <br> | Product is pBca9145-I716216. <br> | ||
| - | Digest pBca9145-Bca1090 (BamHI/ | + | Digest pBca9145-Bca1090 (BamHI/BglI, 1538+553+9bp, Large) <br> |
| - | Digest pBca9145-I716212 (BglII/ | + | Digest pBca9145-I716212 (BglII/BglI, 2187+923bp, Small) <br> |
Product is pBca9145-I716217. <br> | Product is pBca9145-I716217. <br> | ||
| - | Digest pBca1101-Bca1106A (BamHI/ | + | Digest pBca1101-Bca1106A (BamHI/BglI, 1588+850+544bp, Large) <br> |
| - | Digest pBca9145-I716213 (BglII/ | + | Digest pBca9145-I716213 (BglII/BglI, 2187+923bp, Small) <br> |
Product is pBca9145-I716218. <br> | Product is pBca9145-I716218. <br> | ||
| - | Digest pBca1101-Bca1106B (BamHI/ | + | Digest pBca1101-Bca1106B (BamHI/BglI, 1588+850+544bp, Large) <br> |
| - | Digest pBca9145-I716213(BglII/ | + | Digest pBca9145-I716213 (BglII/BglI, 2187+923bp, Small)) <br> |
Product is pBca9145-I716219. <br> | Product is pBca9145-I716219. <br> | ||
| - | Digest pBca1101-Bca1128A (BamHI/ | + | Digest pBca1101-Bca1128A (BamHI/BglI, 1606+850+544bp, Large) <br> |
| - | Digest pBca9145-I716213(BglII/ | + | Digest pBca9145-I716213 (BglII/BglI, 2187+923bp, Small) <br> |
Product is pBca9145-I716220. <br> | Product is pBca9145-I716220. <br> | ||
| - | Digest pBca9145-Bca1090 (BamHI/ | + | Digest pBca9145-Bca1090 (BamHI/BglI, 1538+553+9bp, Large) <br> |
| - | Digest pBca9145-I716213(BglII/ | + | Digest pBca9145-I716213 (BglII/BglI, 2187+923bp, Small)<br> |
Product is pBca9145-I716221. <br> | Product is pBca9145-I716221. <br> | ||
Revision as of 21:12, 5 June 2007
NOT DONE YET...found an ALWNI site in Cre, use BglI instead
[rbs][ATG]
4 Different rbs variants (1106A, 1106B, 1128A, 1090)
2 Different Cre variants (212 and 213)
Digest pBca1101-Bca1106A (BamHI/BglI, 1588+850+544bp, Large)
Digest pBca9145-I716212 (BglII/BglI, 2187+923bp, Small)
Product is pBca9145-I716214.
Digest pBca1101-Bca1106B (BamHI/BglI, 1588+850+544bp, Large)
Digest pBca9145-I716212 (BglII/BglI, 2187+923bp, Small)
Product is pBca9145-I716215.
Digest pBca1101-Bca1128A (BamHI/BglI, 1606+850+544bp, Large)
Digest pBca9145-I716212 (BglII/BglI, 2187+923bp, Small)
Product is pBca9145-I716216.
Digest pBca9145-Bca1090 (BamHI/BglI, 1538+553+9bp, Large)
Digest pBca9145-I716212 (BglII/BglI, 2187+923bp, Small)
Product is pBca9145-I716217.
Digest pBca1101-Bca1106A (BamHI/BglI, 1588+850+544bp, Large)
Digest pBca9145-I716213 (BglII/BglI, 2187+923bp, Small)
Product is pBca9145-I716218.
Digest pBca1101-Bca1106B (BamHI/BglI, 1588+850+544bp, Large)
Digest pBca9145-I716213 (BglII/BglI, 2187+923bp, Small))
Product is pBca9145-I716219.
Digest pBca1101-Bca1128A (BamHI/BglI, 1606+850+544bp, Large)
Digest pBca9145-I716213 (BglII/BglI, 2187+923bp, Small)
Product is pBca9145-I716220.
Digest pBca9145-Bca1090 (BamHI/BglI, 1538+553+9bp, Large)
Digest pBca9145-I716213 (BglII/BglI, 2187+923bp, Small)
Product is pBca9145-I716221.
