BerkiGEM2007-SamanthaConstructionFile8

From 2007.igem.org

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NOT DONE YET...found an ALWNI site in Cre....
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'''[rbs][ATG][lox][term][lox]''' <br>
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'''2 Different terminators (1124 and 1092 - will probably use 1092 in the end)''' <br>
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'''[rbs][ATG]''' <br>
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'''using Bca1090 rbs site''' <br>
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'''4 Different rbs variants (1106A, 1106B, 1128A, 1090)''' <br>
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'''2 Different Cre variants (212 and 213)''' <br>
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----
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Digest pBca1101-Bca1106A (BamHI/AlwnI/Xhol, 1588+850+544bp, Large) <br>
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Digest pBca9145-I716204 (BamHI/AlwnI, 1547+553bp, Large) <br>
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Digest pBca9145-I716212 (BglII/AlwnI, 1519+562bp, Small) <br>
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Digest pBca9145-I716207 (BglII/AlwnI, 1519+797bp, Small) <br>
Product is pBca9145-I716214. <br>
Product is pBca9145-I716214. <br>
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Digest pBca1101-Bca1106B (BamHI/AlwnI/Xhol, 1588+850+544bp, Large) <br>
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Digest pBca9145-I716204 (BamHI/AlwnI, 1547+553bp, Large) <br>
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Digest pBca9145-I716212 (BglII/AlwnI, 1519+562bp, Small) <br>
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Digest pBca9145-I716208 (BglII/AlwnI, 1519+768bp, Small) <br>
Product is pBca9145-I716215. <br>
Product is pBca9145-I716215. <br>
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Digest pBca1101-Bca1128A (BamHI/AlwnI/Xhol, 1606+850+544bp, Large) <br>
 
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Digest pBca9145-I716212 (BglII/AlwnI, 1519+562bp, Small) <br>
 
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Product is pBca9145-I716216. <br>
 
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Digest pBca9145-Bca1090 (BamHI/AlwnI/Xhol, 1538+553+9bp, Large) <br>
 
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Digest pBca9145-I716212 (BglII/AlwnI, 1519+562bp, Small) <br>
 
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Product is pBca9145-I716217. <br>
 
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Digest pBca1101-Bca1106A (BamHI/AlwnI/Xhol, 1588+850+544bp, Large) <br>
 
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Digest pBca9145-I716213 (BglII/AlwnI, 1519+562bp, Small) <br>
 
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Product is pBca9145-I716218. <br>
 
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Digest pBca1101-Bca1106B (BamHI/AlwnI/Xhol, 1588+850+544bp, Large) <br>
 
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Digest pBca9145-I716213(BglII/AlwnI, 1519+562bp, Small) <br>
 
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Product is pBca9145-I716219. <br>
 
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Digest pBca1101-Bca1128A (BamHI/AlwnI/Xhol, 1606+850+544bp, Large) <br>
 
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Digest pBca9145-I716213(BglII/AlwnI, 1519+562bp, Small) <br>
 
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Product is pBca9145-I716220. <br>
 
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Digest pBca9145-Bca1090 (BamHI/AlwnI/Xhol, 1538+553+9bp, Large) <br>
 
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Digest pBca9145-I716213(BglII/AlwnI, 1519+562bp, Small) <br>
 
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Product is pBca9145-I716221. <br>
 

Latest revision as of 18:06, 8 June 2007

[rbs][ATG][lox][term][lox]
2 Different terminators (1124 and 1092 - will probably use 1092 in the end)
using Bca1090 rbs site



Digest pBca9145-I716204 (BamHI/AlwnI, 1547+553bp, Large)
Digest pBca9145-I716207 (BglII/AlwnI, 1519+797bp, Small)
Product is pBca9145-I716214.


Digest pBca9145-I716204 (BamHI/AlwnI, 1547+553bp, Large)
Digest pBca9145-I716208 (BglII/AlwnI, 1519+768bp, Small)
Product is pBca9145-I716215.