BerkiGEM2007-SamanthaConstructionFile8

From 2007.igem.org

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'''[lox][term][lox]''' <br>
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'''[rbs][ATG][lox][term][lox]''' <br>
'''2 Different terminators (1124 and 1092 - will probably use 1092 in the end)''' <br>
'''2 Different terminators (1124 and 1092 - will probably use 1092 in the end)''' <br>
 +
'''using Bca1090 rbs site''' <br>
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----
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Digest pBca9145-I716205 (BamHI/AlwnI, 1723+553bp, Large) <br>
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Digest pBca9145-I716204 (BamHI/AlwnI, 1547+553bp, Large) <br>
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Digest pBca9145-Bca1114 (BglII/AlwnI, 1519+593bp, Small) <br>
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Digest pBca9145-I716207 (BglII/AlwnI, 1519+797bp, Small) <br>
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Product is pBca9145-I716207. <br>
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Product is pBca9145-I716214. <br>
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Digest pBca9145-I716206 (BamHI/AlwnI, 1694+553bp, Large) <br>
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Digest pBca9145-I716204 (BamHI/AlwnI, 1547+553bp, Large) <br>
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Digest pBca9145-Bca1114 (BglII/AlwnI, 1519+593bp, Small) <br>
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Digest pBca9145-I716208 (BglII/AlwnI, 1519+768bp, Small) <br>
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Product is pBca9145-I716208. <br>
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Product is pBca9145-I716215. <br>

Latest revision as of 18:06, 8 June 2007

[rbs][ATG][lox][term][lox]
2 Different terminators (1124 and 1092 - will probably use 1092 in the end)
using Bca1090 rbs site



Digest pBca9145-I716204 (BamHI/AlwnI, 1547+553bp, Large)
Digest pBca9145-I716207 (BglII/AlwnI, 1519+797bp, Small)
Product is pBca9145-I716214.


Digest pBca9145-I716204 (BamHI/AlwnI, 1547+553bp, Large)
Digest pBca9145-I716208 (BglII/AlwnI, 1519+768bp, Small)
Product is pBca9145-I716215.