BerkiGEM2007-SamanthaConstructionFile9

From 2007.igem.org

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'''ATG Cre variants''' <br>
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'''ATG Cre variant''' <br>
Digest pBca1121-Bca9230  (EcoRI/BamHI, 2927+955bp, Small) <br>
Digest pBca1121-Bca9230  (EcoRI/BamHI, 2927+955bp, Small) <br>
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Product is pBca9145-I716224. <br>
Product is pBca9145-I716224. <br>
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Digest pBca1122-Bca9230 (EcoRI/BamHI, 2927+1087bp, Small) <br>
 
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Digest pBca9145-Bca1117 (EcoRI/BglII, 3101+9bp, Large) <br>
 
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Plate on KanAmp<br>
 
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Product is pBca9145-I716225. <br>
 
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'''GTG Cre variant''' <br>
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+
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'''GTG Cre variants''' <br>
+
Digest pBca1121-Bca9230  (EcoRI/BamHI, 2927+955bp, Small) <br>
Digest pBca1121-Bca9230  (EcoRI/BamHI, 2927+955bp, Small) <br>
Digest pBca9145-I716212  (EcoRI/BglII, 3101+9bp, Large)<br>
Digest pBca9145-I716212  (EcoRI/BglII, 3101+9bp, Large)<br>
Plate on CamAmp  <br>
Plate on CamAmp  <br>
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Product is pBca9145-I716226. <br>
+
Product is pBca9145-I716225. <br>
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+
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Digest pBca1122-Bca9230 (EcoRI/BamHI, 2927+1087bp, Small) <br>
+
-
Digest pBca9145-I716212 (EcoRI/BglII, 3101+9bp, Large) <br>
+
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Plate on KanAmp <br>
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Product is pBca9145-I716227. <br>
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Digest pBca9145-I716213  (EcoRI/BglII, 3101+9bp, Large)<br>
Digest pBca9145-I716213  (EcoRI/BglII, 3101+9bp, Large)<br>
Plate on CamAmp <br>
Plate on CamAmp <br>
-
Product is pBca9145-I716228. <br>
+
Product is pBca9145-I716226. <br>
-
 
+
-
Digest pBca1122-Bca9230 (EcoRI/BamHI, 2927+1087bp, Small) <br>
+
-
Digest pBca9145-I716213 (EcoRI/BglII, 3101+9bp, Large) <br>
+
-
Plate on KanAmp <br>
+
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Product is pBca9145-I716229. <br>
+

Latest revision as of 22:50, 20 June 2007

[rbs][Cre]
rbs library - want a weak rbs actually
3 Different Cre variants (ATG Bca1117, GTG I716212 and TTG I716213)


ATG Cre variant

Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small)
Digest pBca9145-Bca1117 (EcoRI/BglII, 3101+9bp, Large)
Plate on CamAmp
Product is pBca9145-I716224.


GTG Cre variant

Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small)
Digest pBca9145-I716212 (EcoRI/BglII, 3101+9bp, Large)
Plate on CamAmp
Product is pBca9145-I716225.


TTG Cre variants

Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small)
Digest pBca9145-I716213 (EcoRI/BglII, 3101+9bp, Large)
Plate on CamAmp
Product is pBca9145-I716226.