BerkiGEM2007-VaibhaviConstructionFile2
From 2007.igem.org
(Difference between revisions)
Revision as of 22:29, 8 June 2007
Construction of a Biobrick 2.0 basic part in plasmid pBca9145-Bca1144 (Clone # 5) for the SodC open reading frame from Escherichia coli K-12 MG1655
PCR VU01F.sodC/VU02R.sodC on SodC (553 bp, BglII/XhoI)
Digest pBca9145-Bca1144 (Clone # 5) (BglII/XhoI, 2063+910, L) Product is pBca9145-VU1 -------------------------------------
VU01F.sodC – ccaaaAGATCTatgaaacgttttagtctggc VU02R.sodC – gctagCTCGAGttaGGATCCttacttaattacaccacaggc
