BerkiGEM2007-VaibhaviConstructionFile2
From 2007.igem.org
(Difference between revisions)
| (One intermediate revision not shown) | |||
| Line 1: | Line 1: | ||
| - | Construction of a Biobrick 2.0 basic part in plasmid pBca9145- | + | Construction of a Biobrick 2.0 basic part in plasmid pBca9145-Bca1145 for the SodC open reading frame from Escherichia coli K-12 MG1655 |
<pre> | <pre> | ||
PCR VU01F.sodC/VU02R.sodC on SodC (553 bp, BglII/XhoI) | PCR VU01F.sodC/VU02R.sodC on SodC (553 bp, BglII/XhoI) | ||
Digest pBca9145-Bca1144 (Clone # 5) (BglII/XhoI, 2063+910, L) | Digest pBca9145-Bca1144 (Clone # 5) (BglII/XhoI, 2063+910, L) | ||
| - | Product is | + | Product is I716252 |
------------------------------------- | ------------------------------------- | ||
VU01F.sodC – ccaaaAGATCTatgaaacgttttagtctggc | VU01F.sodC – ccaaaAGATCTatgaaacgttttagtctggc | ||
VU02R.sodC – gctagCTCGAGttaGGATCCttacttaattacaccacaggc | VU02R.sodC – gctagCTCGAGttaGGATCCttacttaattacaccacaggc | ||
</pre> | </pre> | ||
Latest revision as of 20:35, 27 June 2007
Construction of a Biobrick 2.0 basic part in plasmid pBca9145-Bca1145 for the SodC open reading frame from Escherichia coli K-12 MG1655
PCR VU01F.sodC/VU02R.sodC on SodC (553 bp, BglII/XhoI) Digest pBca9145-Bca1144 (Clone # 5) (BglII/XhoI, 2063+910, L) Product is I716252 ------------------------------------- VU01F.sodC – ccaaaAGATCTatgaaacgttttagtctggc VU02R.sodC – gctagCTCGAGttaGGATCCttacttaattacaccacaggc
