BerkiGEM2007-VaibhaviConstructionFile4
From 2007.igem.org
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| - | Construction of a Biobrick 2.0 basic part in plasmid pBca9145- | + | Construction of a Biobrick 2.0 basic part in plasmid pBca9145-Bca1145 for the N-terminal methionine aminopeptidase (map) open reading frame from Salmonella typhimurium LT2 |
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Latest revision as of 22:43, 8 June 2007
Construction of a Biobrick 2.0 basic part in plasmid pBca9145-Bca1145 for the N-terminal methionine aminopeptidase (map) open reading frame from Salmonella typhimurium LT2
PCR VU07F.map/VU08R.map on methionine aminopeptidase (817 bp, BglII/XhoI) Digest pBca9145-Bca1144 (Clone # 5) (BglII/XhoI, 2063+910, L) Product is pBca9145-VU3 ------------------------------------- VU07F.map ccaaaAGATCTatggctatctcaatcaagacccc VU08R.map gctagCTCGAGttaGGATCCttattcgtcgtgcgagattatcg
