BerkiGEM2007-VaibhaviConstructionFile6

From 2007.igem.org

(Difference between revisions)
 
Line 2: Line 2:
Biobrickification of composite part: RBS (pBca 9145 – Bca1090) + AHSP gene
Biobrickification of composite part: RBS (pBca 9145 – Bca1090) + AHSP gene
---------------------------------------------------
---------------------------------------------------
-
Digest pBca9145-Bca1145 (BglII/BsaI, 1783+970, L)
+
Digest pBca 9145 – Bca1090 (AlwNI/BamHI, 1538 + 553, L)
-
Sub into pBca9145-Bca9205 (BamHI/BsaI/AlwNI, 1049+553+449, L)
+
Sub into I716055 (AlwNI/BglII, 1519 + 868, S)
Product is I716256
Product is I716256
---------------------------------------------------
---------------------------------------------------
No Oligos Needed
No Oligos Needed
</pre>
</pre>

Latest revision as of 20:49, 27 June 2007

Biobrickification of composite part: RBS (pBca 9145 – Bca1090) + AHSP gene
---------------------------------------------------
Digest pBca 9145 – Bca1090		(AlwNI/BamHI, 1538 + 553, L)
Sub into I716055			(AlwNI/BglII, 1519 + 868, S)
Product is I716256
---------------------------------------------------
No Oligos Needed