BerkiGEM2007-VaibhaviConstructionFile7

From 2007.igem.org

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<pre>
<pre>
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Biobrickification of composite Ptet + RFP (product = I716251)
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Biobrickification of composite part: RBS (pBca 9145 – Bca1090) + sodC gene
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Digest pBca9145-Bca1145 (BglII/BsaI, 1783+970, L)
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Digest pBca 9145 – Bca1090 (AlwNI/BamHI, 1538+553, L)
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Sub into pBca9145-Bca9205 (BamHI/BsaI/AlwNI, 1049+553+449, L)
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Sub into I716252 (AlwNI/BglII, 1519+1081, S)
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Product is I716251
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Product is I716257
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No Oligos Needed
No Oligos Needed
</pre>
</pre>

Latest revision as of 20:37, 27 June 2007

Biobrickification of composite part: RBS (pBca 9145 – Bca1090) + sodC gene
---------------------------------------------------
Digest pBca 9145 – Bca1090		(AlwNI/BamHI, 1538+553, L)
Sub into I716252			(AlwNI/BglII, 1519+1081, S)
Product is I716257
---------------------------------------------------
No Oligos Needed
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