BerkiGEM2007 WikiPlaying3

From 2007.igem.org

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<div id="apDiv13">
<div id="apDiv13">
   <div align="center">
   <div align="center">
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     <p><span class="style2"><strong><a TITLE="To enable preservation of our bacteria for long time periods, we are including the ability to produce the compounds hydroxyectoine and trehalose that will enable our bacteria to survive freeze-drying intact.  This will dramatically increase shelf-life and decrease transport costs." href="https://2007.igem.org/Berkeley_UC_Storage"><span class="style21">Storage</span><br />
+
     <p><span class="style2"><strong><a TITLE="To enable preservation of our bacteria for long time periods." href="https://2007.igem.org/Berkeley_UC_Storage"><span class="style21">Storage</span><br />
-
     </a></strong></span><a TITLE="To enable preservation of our bacteria for long time periods, we are including the ability to produce the compounds hydroxyectoine and trehalose that will enable our bacteria to survive freeze-drying intact.  This will dramatically increase shelf-life and decrease transport costs." href="https://2007.igem.org/Berkeley_UC_Storage"><img src="https://static.igem.org/mediawiki/2007/7/7a/TrehaloseButton.gif" alt="" name="TrehaloseButton" width="75" height="75" id="TrehaloseButton" /></a></p>
+
     </a></strong></span><a TITLE="To enable preservation of our bacteria for long time periods." href="https://2007.igem.org/Berkeley_UC_Storage"><img src="https://static.igem.org/mediawiki/2007/7/7a/TrehaloseButton.gif" alt="" name="TrehaloseButton" width="75" height="75" id="TrehaloseButton" /></a></p>
   </div>
   </div>
</div>
</div>
<div id="apDiv16">
<div id="apDiv16">
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   <div align="center"><span class="style27"><a TITLE="To prevent chance of infection or unwanted proliferation after hemoglobin production, we have engineered a genetic self-destruct mechanism whereby when induced, the bacterial cell will express a genetic material-degrading toxin which kills the cell, but leaves it physically intact." href="https://2007.igem.org/Berkeley_UC_KillSwitch" class="style22">Genetic Self Destruct</a></span><a TITLE="To prevent chance of infection or unwanted proliferation after hemoglobin production, we have engineered a genetic self-destruct mechanism whereby when induced, the bacterial cell will express a genetic material-degrading toxin which kills the cell, but leaves it physically intact." href="https://2007.igem.org/Berkeley_UC_KillSwitch"><img src="https://static.igem.org/mediawiki/2007/f/ff/KillSwitchButton.gif" alt="" name="KillSwitchButton" width="75" height="75" id="KillSwitchButton" /></a></div>
+
   <div align="center"><span class="style27"><a TITLE="To prevent chance of infection or unwanted proliferation after hemoglobin production." href="https://2007.igem.org/Berkeley_UC_KillSwitch" class="style22">Genetic Self Destruct</a></span><a TITLE="To prevent chance of infection or unwanted proliferation after hemoglobin production." href="https://2007.igem.org/Berkeley_UC_KillSwitch"><img src="https://static.igem.org/mediawiki/2007/f/ff/KillSwitchButton.gif" alt="" name="KillSwitchButton" width="75" height="75" id="KillSwitchButton" /></a></div>
</div>
</div>
<div id="apDiv20">
<div id="apDiv20">
-
   <div align="center"><span class="style27"><a href="https://2007.igem.org/Berkeley_UC_GrowthControl" class="style22">Growth Control</a></span><a href="https://2007.igem.org/Berkeley_UC_GrowthControl"><img src="https://static.igem.org/mediawiki/2007/b/bf/DNADeletionButton.gif" alt="" name="GrowthControlButton" width="75" height="75" id="GrowthControlButton" /></a></div>
+
   <div align="center"><span class="style27"><a TITLE="Growth aided by antioxidants in vitro and inhibited in vivo by lack of free iron." href="https://2007.igem.org/Berkeley_UC_GrowthControl" class="style22">Growth Control</a></span><a TITLE="Growth aided by antioxidants in vitro and inhibited in vivo by lack of free iron." href="https://2007.igem.org/Berkeley_UC_GrowthControl"><img src="https://static.igem.org/mediawiki/2007/b/bf/DNADeletionButton.gif" alt="" name="GrowthControlButton" width="75" height="75" id="GrowthControlButton" /></a></div>
</div>
</div>
<div id="apDiv6">
<div id="apDiv6">
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</div>
</div>
<div id="apDiv11">
<div id="apDiv11">
-
   <div align="center"><strong><span class="style21"><a TITLE="Our bacterial chassis has been modified to resist destruction by the immune system." class="style22"> Chassis</a> </span><br />
+
   <div align="center"><strong><span class="style21"><a href="https://2007.igem.org/Berkeley_UC_Immunity_Chassis" TITLE="Our bacterial chassis has been modified to resist destruction by the immune system." class="style22"> Chassis</a> </span><br />
   </strong><a TITLE="Our bacterial chassis has been heavily modified to remove its sepsis-inducing toxicity, immunogenic factors, and ability to grow within the bloodstream, as well as promote its ability to last longer in the bloodstream by masking it from the immune system." href="https://2007.igem.org/Berkeley_UC_Immunity"><img src="https://static.igem.org/mediawiki/2007/f/f9/ImmunityButton.gif" alt="" name="ImmunityButton" width="100" height="75" id="ImmunityButton" TITLE="Our bacterial chassis has been modified to resist destruction by the immune system." href="https://2007.igem.org/Berkeley_UC_Immunity"" /></a></div>
   </strong><a TITLE="Our bacterial chassis has been heavily modified to remove its sepsis-inducing toxicity, immunogenic factors, and ability to grow within the bloodstream, as well as promote its ability to last longer in the bloodstream by masking it from the immune system." href="https://2007.igem.org/Berkeley_UC_Immunity"><img src="https://static.igem.org/mediawiki/2007/f/f9/ImmunityButton.gif" alt="" name="ImmunityButton" width="100" height="75" id="ImmunityButton" TITLE="Our bacterial chassis has been modified to resist destruction by the immune system." href="https://2007.igem.org/Berkeley_UC_Immunity"" /></a></div>
</div>
</div>
<div id="apDiv14">
<div id="apDiv14">
-
   <div align="center"><span class="style27"><a TITLE="The Controller is an integrated genetic circuit comprised of two plasmids that allows stable maintenance of the system's various operons on a large single-copy plasmid in a dormant state. Upon induction, the copy number of the operons and their transcription increase 100-fold resulting in a dramatic increase in protein expression." href="https://2007.igem.org/Berkeley_UC_SystemController" class="style22">System Controller</a></span><a TITLE="The Controller is an integrated genetic circuit comprised of two plasmids that allows stable maintenance of the system's various operons on a large single-copy plasmid in a dormant state. Upon induction, the copy number of the operons and their transcription increase 100-fold resulting in a dramatic increase in protein expression." href="https://2007.igem.org/Berkeley_UC_SystemController"><img src="https://static.igem.org/mediawiki/2007/c/cd/2PlasmidButton.gif" alt="" name="ControllerButton" width="75" height="75" id="ControllerButton" /></a></div>
+
   <div align="center"><span class="style27"><a TITLE="The Controller is an integrated genetic circuit comprised of two plasmids." href="https://2007.igem.org/Berkeley_UC_SystemController" class="style22">System Controller</a></span><a TITLE="The Controller is an integrated genetic circuit comprised of two plasmids." href="https://2007.igem.org/Berkeley_UC_SystemController"><img src="https://static.igem.org/mediawiki/2007/c/cd/2PlasmidButton.gif" alt="" name="ControllerButton" width="75" height="75" id="ControllerButton" /></a></div>
</div>
</div>
<div id="apDiv36">
<div id="apDiv36">
-
   <div align="center"><span class="style5"><a href="https://2007.igem.org/Berkeley_UC_Introduction">Project Tour<br />
+
   <div align="center"><span class="style5"><a href="https://2007.igem.org/Berkeley_UC_Introduction"><span class="style30">Project Tour</span><br />
   </a></span><a href="https://2007.igem.org/Berkeley_UC_Introduction"><img src="https://static.igem.org/mediawiki/2007/a/a0/F-Blue.gif" alt="" name="UCForwardButton" width="54" height="41" id="UCForwardButton" /></a></div>
   </a></span><a href="https://2007.igem.org/Berkeley_UC_Introduction"><img src="https://static.igem.org/mediawiki/2007/a/a0/F-Blue.gif" alt="" name="UCForwardButton" width="54" height="41" id="UCForwardButton" /></a></div>
</div>
</div>
<div id="apDiv52">
<div id="apDiv52">
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  <font color="#FFFF00">
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  <div align="center">
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    <p align="right"><span class="style28 style44"><strong><span class="style76">
-
 
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      <br>
-
 
+
      <span class="style77 style28">Team Notebooks</span>
-
 
+
      </li>
-
 
+
     
-
 
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        <a href="https://2007.igem.org/John_Dueber_Notebook"><font color="#FFFF00"></a></span></strong></span></p>
-
 
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    <p align="right" class="style47"><a href="https://2007.igem.org/John_Dueber_Notebook"><font color="#FFFF00"><span class="style29">John Dueber</span></a></p>
-
 
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    <p align="right" class="style44"><a href="https://2007.igem.org/Christopher_Anderson_Notebook"><font color="#FFFF00">Christopher Anderson</a></p>
-
 
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    <p align="right" class="style44"><a href="https://2007.igem.org/Farnaz_Nowroozi_Notebook"><font color="#FFFF00">Farnaz Nowroozi</a></p>
-
 
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    <p align="right" class="style44"><a href="https://2007.igem.org/Amin_Hajimorad_Notebook"><font color="#FFFF00">Amin Hajimora</a></p>
-
 
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    <p align="right" class="style44"><a href="https://2007.igem.org/Rickey_Bonds_Notebook"><font color="#FFFF00">Ricky Bonds</a></p>
-
 
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    <p align="right" class="style44"><a href="https://2007.igem.org/Arthur_Yu_Notebook"><font color="#FFFF00">Arthur Yu</a></p>
-
 
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    <p align="right" class="style44"><a href="https://2007.igem.org/Austin_Day_Notebook"><font color="#FFFF00">Austin Day</a></p>
-
 
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    <p align="right" class="style44"><a href="https://2007.igem.org/David_Tulga_Notebook" class="style43"><font color="#FFFF00">David Tulga</a></p>
-
 
+
    <p align="right" class="style44"><a href="https://2007.igem.org/Kristin_Doan_Notebook"><font color="#FFFF00">Kristin Doan</a></p>
-
 
+
    <p align="right" class="style44"><a href="https://2007.igem.org/Samantha_Liang_Notebook"><font color="#FFFF00">Samantha Liang 1</a></p>
-
 
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    <p align="right" class="style44"><a href="https://2007.igem.org/Samantha_Liang_Notebook2"><font color="#FFFF00">Samantha Liang 2</a></p>
-
 
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    <p align="right" class="style44"><a href="https://2007.igem.org/Vaibhavi_Umesh_Notebook"><font color="#FFFF00">Vaibhavi Umesh</a></p>
-
 
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    <p align="right" class="style44"><a href="https://2007.igem.org/Kristin_Fuller_Notebook"><font color="#FFFF00">Kristin Fuller</a></p>
 +
    <p align="right" class="style44"><a href="https://2007.igem.org/Vincent_Parker_Notebook"><font color="#FFFF00">Vincent Parker</a></p>
 +
    <p align="right" class="style44"><a href="https://2007.igem.org/Nhu_Nguyen_Notebook"><font color="#FFFF00">Nhu Nguyen</a></p>
 +
    <p align="right" class="style47"><span class="style29"><strong><a href="https://2007.igem.org/Hannah_Cole_Notebook" class="style44"><font color="#FFFF00">Hannah Cole</a></strong></span></p>
 +
  </div>
</div>
</div>
<div class="style12" id="apDiv50">
<div class="style12" id="apDiv50">
   <div class="style24">
   <div class="style24">
-
     <p class="style28"><span class="style25"><a href="https://2007.igem.org/Berkeley_UC_Introduction">Introduction</a><br>
+
     <p class="style28"><span class="style25"><a href="https://2007.igem.org/Berkeley_UC_Introduction">Introduction</a></span><a href="https://2007.igem.org/Berkeley_UC_OxygenTransport" TITLE="Hemoglobin production and maintenance is essential for oxygen delivery."><br>
-
    </span><a TITLE="Hemoglobin production and maintenance is essential for oxygen delivery."><br>
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     <span class="style31">Oxygen Transport </span></a> </p>
-
     Oxygen Transport </a> </p>
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     <ul>
     <ul>
       <li class="style29"><span class="style29"><a TITLE="Human hemoglobin alpha is our choice of oxygen carrier." href="https://2007.igem.org/Berkeley_UC_OxygenTransport_Hemoglobin">Hemoglobin</a></span></li>
       <li class="style29"><span class="style29"><a TITLE="Human hemoglobin alpha is our choice of oxygen carrier." href="https://2007.igem.org/Berkeley_UC_OxygenTransport_Hemoglobin">Hemoglobin</a></span></li>
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           <li class="style29"><span class="style30"><a TITLE="Heme is the oxygen binding component of hemoglobin." href="https://2007.igem.org/Berkeley_UC_OxygenTransport_Chaperones_Heme">Heme</a></span></li>
           <li class="style29"><span class="style30"><a TITLE="Heme is the oxygen binding component of hemoglobin." href="https://2007.igem.org/Berkeley_UC_OxygenTransport_Chaperones_Heme">Heme</a></span></li>
           <li><span class="style30"><a TITLE="Alpha hemoglobin stabilizing protein (AHSP) helps hemoglobin fold properly." href="https://2007.igem.org/Berkeley_UC_OxygenTransport_Chaperones_AHSP">AHSP</a></span></li>
           <li><span class="style30"><a TITLE="Alpha hemoglobin stabilizing protein (AHSP) helps hemoglobin fold properly." href="https://2007.igem.org/Berkeley_UC_OxygenTransport_Chaperones_AHSP">AHSP</a></span></li>
-
           <li><span class="style30"><a TITLE="Cytochrome b5 and b5 reductase help to keep the hemoglobin in its functional form." href="https://2007.igem.org/Berkeley_UC_OxygenTransport_Chaperones_AHSP">Cytochrome b5</a></span></li>
+
           <li><span class="style30"><a TITLE="Cytochrome b5 and b5 reductase help to keep the hemoglobin in its functional form." href="https://2007.igem.org/Berkeley_UC_OxygenTransport_Chaperones_Cytochromes">Cytochrome b5</a></span></li>
-
           <li><span class="style30"><a TITLE="Cytochrome b5 and b5 reductase help to keep the hemoglobin in its functional form." href="https://2007.igem.org/Berkeley_UC_OxygenTransport_Chaperones_AHSP">Cytochrome b5 Reductase</a></span><a href="https://2007.igem.org/Berkeley_UC_OxygenTransport_Chaperones_AHSP"><br />
+
           <li><span class="style30"><a TITLE="Cytochrome b5 and b5 reductase help to keep the hemoglobin in its functional form." href="https://2007.igem.org/Berkeley_UC_OxygenTransport_Chaperones_Cytochromes">Cytochrome b5 Reductase</a></span><a href="https://2007.igem.org/Berkeley_UC_OxygenTransport_Chaperones_AHSP"><br />
                                 </a> </li>
                                 </a> </li>
         </ul>
         </ul>
       </li>
       </li>
     </ul>
     </ul>
-
     <a TITLE="Our bacterial chassis has been heavily modified to remove its sepsis-inducing toxicity, immunogenic factors, and ability to grow within the bloodstream, as well as promote its ability to last longer in the bloodstream by masking it from the immune system." href="https://2007.igem.org/Berkeley_UC_Immunity" class="style28">Immunity</a>     
+
     <a href="https://2007.igem.org/Berkeley_UC_Immunity" TITLE="Our bacterial chassis has been heavily modified to remove its sepsis-inducing toxicity, immunogenic factors, and ability to grow within the bloodstream, as well as promote its ability to last longer in the bloodstream by masking it from the immune system." class="style31">Immunity</a>     
     <ul class="style29">
     <ul class="style29">
-
       <li><a TITLE="Our bacterial chassis has been modified to resist destruction by the immune system.">Chassis</a></li>
+
       <li><a href="https://2007.igem.org/Berkeley_UC_Immunity_Chassis" TITLE="Our bacterial chassis has been modified to resist destruction by the immune system.">Chassis</a></li>
       <li><a TITLE="A deletion of the msbB gene allows our chassis to be less immunogenic." href="https://2007.igem.org/Berkeley_UC_Immunity_msbBDeletion">msbB Deletion</a></li>
       <li><a TITLE="A deletion of the msbB gene allows our chassis to be less immunogenic." href="https://2007.igem.org/Berkeley_UC_Immunity_msbBDeletion">msbB Deletion</a></li>
     </ul>
     </ul>
-
     <p><a TITLE="Growth is engineered to only occur in vitro." href="https://2007.igem.org/Berkeley_UC_GrowthControl" class="style28">Growth Control</a></p>
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     <p><a href="https://2007.igem.org/Berkeley_UC_GrowthControl" TITLE="Growth aided by antioxidants in vitro and inhibited in vivo by lack of free iron." class="style31">Growth Control</a></p>
-
     <ul class="style29"><li><a TITLE="Antioxidants help to prevent damage from superoxide radicals." href="https://2007.igem.org/Berkeley_UC_Antioxidants" class="style29">Antioxidants</a>  
+
     <ul class="style29"><li><a TITLE="Antioxidants help to prevent damage by superoxide radicals." href="https://2007.igem.org/Berkeley_UC_Antioxidants" class="style29">Antioxidants</a>  
       <ul>
       <ul>
-
         <li><span class="style30"><a TITLE="Antioxidants help to prevent damage from superoxide radicals." href="https://2007.igem.org/Berkeley_UC_Antioxidants_SodC">SodC</a></span></li>
+
         <li><span class="style30"><a TITLE="Antioxidants help to prevent damage by superoxide radicals." href="https://2007.igem.org/Berkeley_UC_Antioxidants">SodC</a></span></li>
-
         <li><span class="style30"><a TITLE="Antioxidants help to prevent damage from superoxide radicals." href="https://2007.igem.org/Berkeley_UC_Antioxidants_KatG">KatG</a></span></li>
+
         <li><span class="style30"><a TITLE="Antioxidants help to prevent damage by superoxide radicals." href="https://2007.igem.org/Berkeley_UC_Antioxidants">KatG</a></span></li>
         </ul>
         </ul>
     </li>
     </li>
      
      
-
      <li></li>
+
       <li><a href="https://2007.igem.org/Berkeley_UC_GrowthControl_tonBDeletion" TITLE="TonB is essential for high affinity iron uptake." class="style29">TonB Deletion</a><a href="https://2007.igem.org/Berkeley_UC_GrowthControl_tonBDeletion"><br>
-
       <li><a TITLE="TonB is essential for high affinity iron uptake." href="https://2007.igem.org/Berkeley_UC_GrowthControl_tonBDeletion" class="style29">TonB Deletion</a><a href="https://2007.igem.org/Berkeley_UC_GrowthControl_tonBDeletion"><br>
+
       </a></li>
       </a></li>
     </ul>
     </ul>
-
     <p><span class="style28"><a href="https://2007.igem.org/Berkeley_UC_KillSwitch" TITLE="To prevent chance of infection or unwanted proliferation after hemoglobin production." class="style28">Genetic Self Destruct</a></span></p>
+
     <p><span class="style28"><span class="style31"><a href="https://2007.igem.org/Berkeley_UC_KillSwitch" TITLE="To prevent chance of infection or unwanted proliferation after hemoglobin production." class="style31">Genetic Self Destruct</a></span></span></p>
-
     <p class="style28"><a TITLE="The Controller is an integrated genetic circuit comprised of two plasmids that allows stable maintenance of the system's various operons on a large single-copy plasmid in a dormant state. Upon induction, the copy number of the operons and their transcription increase 100-fold resulting in a dramatic increase in protein expression." href="https://2007.igem.org/Berkeley_UC_SystemController">SystemController</a></p>
+
     <p class="style31"><a TITLE="The Controller is an integrated genetic circuit comprised of two plasmids." href="https://2007.igem.org/Berkeley_UC_SystemController">SystemController</a></p>
-
     <span class="style28"><a TITLE="To enable preservation of our bacteria for long time periods, we are including the ability to produce the compounds hydroxyectoine and trehalose that will enable our bacteria to survive freeze-drying intact.  This will dramatically increase shelf-life and decrease transport costs." href="https://2007.igem.org/Berkeley_UC_Storage" class="style28">Storage</a></span></div>
+
     <span class="style31"><a TITLE="To enable preservation of our bacteria for long time periods, we are including the ability to produce the compounds hydroxyectoine and trehalose that will enable our bacteria to survive freeze-drying intact.  This will dramatically increase shelf-life and decrease transport costs." href="https://2007.igem.org/Berkeley_UC_Storage" class="style31">Storage</a></span></div>
   <p class="style17">&nbsp;</p>
   <p class="style17">&nbsp;</p>
   <p class="style17">&nbsp;</p>
   <p class="style17">&nbsp;</p>
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