From 2007.igem.org

Revision as of 13:29, 20 September 2007 (view source) Robertina (Talk | contribs) ← Older edit		Revision as of 13:31, 20 September 2007 (view source) Robertina (Talk | contribs) Newer edit →
Line 1:		Line 1:
	FOX3 XbaI Fw		FOX3 XbaI Fw
		+
	5’- GC TCTAGA AGTATGTCGTGCTCGAAGTCA -3’		5’- GC TCTAGA AGTATGTCGTGCTCGAAGTCA -3’
		+
	Lungh=21		Lungh=21
		+
	Tm 60°C		Tm 60°C
	FOX3 SpeI Rev		FOX3 SpeI Rev
		+
	5’- GG ACTAGA TGCTTTTATGTAGTGTTAT-3’		5’- GG ACTAGA TGCTTTTATGTAGTGTTAT-3’
		+
	Lungh=19		Lungh=19
		+
	Tm 50°C		Tm 50°C
-	LUCIFERASE Xba I  Fv	+	Luciferase Xba I  Fv
		+
	5’- GC TCTAGA ATGGAAGACGCCAAAAACATAA-3’		5’- GC TCTAGA ATGGAAGACGCCAAAAACATAA-3’
		+
	Lungh=22		Lungh=22
		+
	Tm 60°C		Tm 60°C
-	LUCIFERASE   Spe I Rv	+	Luciferase   Spe I Rv
		+
	5’- GG ACTAGA TTAAAGCTTCTTTCCGCCCTT-3’		5’- GG ACTAGA TTAAAGCTTCTTTCCGCCCTT-3’
		+
	Lungh=20		Lungh=20
		+
	Tm 60°C		Tm 60°C
	2XORE – CYC1 XbaI  Fw		2XORE – CYC1 XbaI  Fw
		+
	5’-GC TCTAGA ATCAGTTATTACCCTCGACCTC-3’		5’-GC TCTAGA ATCAGTTATTACCCTCGACCTC-3’
		+
	Lungh=22		Lungh=22
		+
	Tm 58,4°C		Tm 58,4°C
	1X ORE – CYC1 XbaI Fw		1X ORE – CYC1 XbaI Fw
		+
	5’- GC TCTAGA GAGATGGCTCGACCATCT-3’		5’- GC TCTAGA GAGATGGCTCGACCATCT-3’
		+
	Lungh=18		Lungh=18
		+
	Tm 56°C		Tm 56°C
	2&1ORE-CYC1 Spe I Rev		2&1ORE-CYC1 Spe I Rev
		+
	5’- GG ACTAGA GCCATGGCCCATCGAATTC-3’		5’- GG ACTAGA GCCATGGCCCATCGAATTC-3’
		+
	Lungh=18		Lungh=18
		+
	Tm 56°C		Tm 56°C

---

Revision as of 13:31, 20 September 2007

FOX3 XbaI Fw

5’- GC TCTAGA AGTATGTCGTGCTCGAAGTCA -3’

Lungh=21

Tm 60°C

FOX3 SpeI Rev

5’- GG ACTAGA TGCTTTTATGTAGTGTTAT-3’

Lungh=19

Tm 50°C

Luciferase Xba I Fv

5’- GC TCTAGA ATGGAAGACGCCAAAAACATAA-3’

Lungh=22

Tm 60°C

Luciferase Spe I Rv

5’- GG ACTAGA TTAAAGCTTCTTTCCGCCCTT-3’

Lungh=20

Tm 60°C

2XORE – CYC1 XbaI Fw

5’-GC TCTAGA ATCAGTTATTACCCTCGACCTC-3’

Lungh=22

Tm 58,4°C

1X ORE – CYC1 XbaI Fw

5’- GC TCTAGA GAGATGGCTCGACCATCT-3’

Lungh=18

Tm 56°C

2&1ORE-CYC1 Spe I Rev

5’- GG ACTAGA GCCATGGCCCATCGAATTC-3’

Lungh=18

Tm 56°C