Biobrick Primers Design

From 2007.igem.org

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'''''FOX3 XbaI Fw'''''
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                                    [[Image:Primers.jpg]]
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5’- GC TCTAGA AGTATGTCGTGCTCGAAGTCA -3’
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'''''FOX3 XbaI Fw'''''
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Lungh=21
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5’- GC TCTAGA AGTATGTCGTGCTCGAAGTCA -3’                                                 
Tm 60°C
Tm 60°C
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''FOX3 SpeI Rev''  
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'''''FOX3 SpeI Rev''  
5’- GG ACTAGA TGCTTTTATGTAGTGTTAT-3’
5’- GG ACTAGA TGCTTTTATGTAGTGTTAT-3’
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Lungh=19
 
Tm 50°C
Tm 50°C
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''Luciferase Xba I  Fv''   
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'''''Luciferase Xba I  Fv'''''   
5’- GC TCTAGA ATGGAAGACGCCAAAAACATAA-3’       
5’- GC TCTAGA ATGGAAGACGCCAAAAACATAA-3’       
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Lungh=22
 
Tm 60°C
Tm 60°C
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''Luciferase  Spe I Rv''
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'''''Luciferase  Spe I Rv'' '''                                                   
5’- GG ACTAGA TTAAAGCTTCTTTCCGCCCTT-3’
5’- GG ACTAGA TTAAAGCTTCTTTCCGCCCTT-3’
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Lungh=20
 
Tm 60°C
Tm 60°C
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2XORE – CYC1 XbaI  Fw
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'''''2XORE – CYC1 XbaI  Fw'''''
5’-GC TCTAGA ATCAGTTATTACCCTCGACCTC-3’
5’-GC TCTAGA ATCAGTTATTACCCTCGACCTC-3’
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Lungh=22
 
Tm 58,4°C
Tm 58,4°C
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''1X ORE – CYC1 XbaI Fw''
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'''''1X ORE – CYC1 XbaI Fw'''''
5’- GC TCTAGA GAGATGGCTCGACCATCT-3’
5’- GC TCTAGA GAGATGGCTCGACCATCT-3’
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Lungh=18
 
Tm 56°C
Tm 56°C
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''2&1ORE-CYC1 Spe I Rev''
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'''''2&1ORE-CYC1 Spe I Rev'''''
5’- GG ACTAGA GCCATGGCCCATCGAATTC-3’
5’- GG ACTAGA GCCATGGCCCATCGAATTC-3’
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Lungh=18
 
Tm 56°C
Tm 56°C

Latest revision as of 11:20, 21 October 2007

                                    Primers.jpg

FOX3 XbaI Fw

5’- GC TCTAGA AGTATGTCGTGCTCGAAGTCA -3’

Tm 60°C

FOX3 SpeI Rev

5’- GG ACTAGA TGCTTTTATGTAGTGTTAT-3’

Tm 50°C

Luciferase Xba I Fv

5’- GC TCTAGA ATGGAAGACGCCAAAAACATAA-3’

Tm 60°C

Luciferase Spe I Rv

5’- GG ACTAGA TTAAAGCTTCTTTCCGCCCTT-3’

Tm 60°C

2XORE – CYC1 XbaI Fw

5’-GC TCTAGA ATCAGTTATTACCCTCGACCTC-3’

Tm 58,4°C

1X ORE – CYC1 XbaI Fw

5’- GC TCTAGA GAGATGGCTCGACCATCT-3’

Tm 56°C

2&1ORE-CYC1 Spe I Rev

5’- GG ACTAGA GCCATGGCCCATCGAATTC-3’

Tm 56°C