Biobrick Primers Design

From 2007.igem.org

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                                    [[Image:Primers.jpg]]
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'''''FOX3 XbaI Fw'''''  
'''''FOX3 XbaI Fw'''''  
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5’- GC TCTAGA AGTATGTCGTGCTCGAAGTCA -3’
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5’- GC TCTAGA AGTATGTCGTGCTCGAAGTCA -3’                                                
Tm 60°C
Tm 60°C
-
''''''''FOX3 SpeI Rev''  
+
'''''FOX3 SpeI Rev''  
5’- GG ACTAGA TGCTTTTATGTAGTGTTAT-3’
5’- GG ACTAGA TGCTTTTATGTAGTGTTAT-3’
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Tm 60°C
Tm 60°C
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'''''Luciferase  Spe I Rv'' '''  
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'''''Luciferase  Spe I Rv'' '''                                                    
5’- GG ACTAGA TTAAAGCTTCTTTCCGCCCTT-3’
5’- GG ACTAGA TTAAAGCTTCTTTCCGCCCTT-3’
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Tm 56°C
Tm 56°C
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[[Image:Primers.jpg]]
 

Latest revision as of 11:20, 21 October 2007

                                    Primers.jpg

FOX3 XbaI Fw

5’- GC TCTAGA AGTATGTCGTGCTCGAAGTCA -3’

Tm 60°C

FOX3 SpeI Rev

5’- GG ACTAGA TGCTTTTATGTAGTGTTAT-3’

Tm 50°C

Luciferase Xba I Fv

5’- GC TCTAGA ATGGAAGACGCCAAAAACATAA-3’

Tm 60°C

Luciferase Spe I Rv

5’- GG ACTAGA TTAAAGCTTCTTTCCGCCCTT-3’

Tm 60°C

2XORE – CYC1 XbaI Fw

5’-GC TCTAGA ATCAGTTATTACCCTCGACCTC-3’

Tm 58,4°C

1X ORE – CYC1 XbaI Fw

5’- GC TCTAGA GAGATGGCTCGACCATCT-3’

Tm 56°C

2&1ORE-CYC1 Spe I Rev

5’- GG ACTAGA GCCATGGCCCATCGAATTC-3’

Tm 56°C